| http://purl.uniprot.org/citations/2526016 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2526016 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2526016 | http://www.w3.org/2000/01/rdf-schema#comment | "Anti-Sm antibodies from a patient with systemic lupus erythematosus (SLE) were used to isolate cDNA clones encoding the snRNP-associated protein N from a rat brain derived cDNA library. The predicted primary structure of the 240 amino acid protein has a proline rich carboxyl terminus and shares a region of sequence similarity with other snRNP polypeptides, A and B/B'. Anti-Sm sera recognize a beta-galactosidase fusion protein containing only the carboxyl-terminal 80 amino acids of N; antibodies eluted from this fusion protein also react with A, B/B' and N on immunoblots, suggesting that these proteins share an Sm epitope located within this segment. Polyclonal antibodies raised against a 23 amino acid synthetic peptide derived from this conserved region of N recognize A, N and B/B' on immunoblots and can immunoprecipitate the Sm class of U snRNAs. These results confirm that this sequence defines a potential Sm epitope. RNA blotting analyses demonstrate that a 1.6 kb mRNA expressed predominantly in brain encodes the N polypeptide in both rats and humans. At low stringency rat N cDNA also hybridizes to a 1.3 kb mRNA species which encodes B/B', suggesting that N is structurally related to, but distinct from B/B'. Although B/B' proteins are thought to be expressed in all human cells, only N and B, but not B', are observed on immunoblots of human brain proteins probed with anti-Sm sera. The apparent difference in the complement of proteins associated with snRNP particles in human brain versus elsewhere suggests a possible mechanism for the regulation of brain-specific mRNA splicing."xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.org/dc/terms/identifier | "doi:10.1002/j.1460-2075.1989.tb03489.x"xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.org/dc/terms/identifier | "doi:10.1002/j.1460-2075.1989.tb03489.x"xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/author | "McAllister G."xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/author | "McAllister G."xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/author | "Amara S.G."xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/author | "Amara S.G."xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/author | "Lerner M.R."xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/author | "Lerner M.R."xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/author | "Roby-Shemkovitz A."xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/author | "Roby-Shemkovitz A."xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/pages | "1177-1181"xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/pages | "1177-1181"xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/title | "cDNA sequence of the rat U snRNP-associated protein N: description of a potential Sm epitope."xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/title | "cDNA sequence of the rat U snRNP-associated protein N: description of a potential Sm epitope."xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/2526016 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2526016 |
| http://purl.uniprot.org/citations/2526016 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2526016 |