| http://purl.uniprot.org/citations/25274630 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25274630 | http://www.w3.org/2000/01/rdf-schema#comment | "Srp1 (importin-α) can translocate proteins that contain a nuclear localization signal (NLS) into the nucleus. The loss of Srp1 is lethal, although several temperature-sensitive mutants have been described. Among these mutants, srp1-31 displays the characteristic nuclear import defect of importin-α mutants, whereas srp1-49 shows a defect in protein degradation. We characterized these and additional srp1 mutants to determine whether distinct mechanisms were required for intracellular proteolysis and the import of NLS-containing proteins. We determined that srp1 mutants that failed to import NLS-containing proteins (srp1-31 and srp1-55) successfully localized proteasomes to the nucleus. In contrast, srp1 mutants that did not target proteasomes to the nucleus (srp1-49 and srp1-E402Q) were able to import NLS-containing proteins. The proteasome targeting defect of specific srp1 mutants caused stabilization of nuclear substrates and overall accumulation of multiubiquitylated proteins. Co-expression of a member of each class of srp1 mutants corrected both the proteasome localization defect and the import of NLS-containing proteins. These findings indicate that the targeting of proteasomes to the nucleus occurs by a mechanism distinct from the Srp1-mediated import of nuclear proteins."xsd:string |
| http://purl.uniprot.org/citations/25274630 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m114.582023"xsd:string |
| http://purl.uniprot.org/citations/25274630 | http://purl.uniprot.org/core/author | "Chen L."xsd:string |
| http://purl.uniprot.org/citations/25274630 | http://purl.uniprot.org/core/author | "Madura K."xsd:string |
| http://purl.uniprot.org/citations/25274630 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25274630 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/25274630 | http://purl.uniprot.org/core/pages | "32339-32352"xsd:string |
| http://purl.uniprot.org/citations/25274630 | http://purl.uniprot.org/core/title | "Yeast importin-alpha (Srp1) performs distinct roles in the import of nuclear proteins and in targeting proteasomes to the nucleus."xsd:string |
| http://purl.uniprot.org/citations/25274630 | http://purl.uniprot.org/core/volume | "289"xsd:string |
| http://purl.uniprot.org/citations/25274630 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25274630 |
| http://purl.uniprot.org/citations/25274630 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25274630 |
| http://purl.uniprot.org/uniprot/Q02821#attribution-529A489855E9BBAF898AD99C4B11472B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/25274630 |
| http://purl.uniprot.org/uniprot/#_A0A6A5Q590-mappedCitation-25274630 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25274630 |
| http://purl.uniprot.org/uniprot/#_P38637-mappedCitation-25274630 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25274630 |
| http://purl.uniprot.org/uniprot/#_Q02821-mappedCitation-25274630 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25274630 |
| http://purl.uniprot.org/uniprot/A0A6A5Q590 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25274630 |
| http://purl.uniprot.org/uniprot/Q02821 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25274630 |
| http://purl.uniprot.org/uniprot/P38637 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25274630 |