| http://purl.uniprot.org/citations/25281742 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25281742 | http://www.w3.org/2000/01/rdf-schema#comment | "Tissue factor (TF) binds the serine protease factor VIIa (FVIIa) to form a proteolytically active complex that can trigger coagulation or activate cell signaling. Here we addressed the involvement of tyrosine kinase receptors (RTKs) in TF/FVIIa signaling by antibody array analysis and subsequently found that EphB2 and EphA2 of the Eph RTK family were cleaved in their ectodomains by TF/FVIIa. We used N-terminal Edman sequencing and LC-MS/MS analysis to characterize the cleaved Eph isoforms and identified a key arginine residue at the cleavage site, in agreement with the tryptic serine protease activity of FVIIa. Protease-activated receptor 2 (PAR2) signaling and downstream coagulation activity was non-essential in this context, in further support of a direct cleavage by TF/FVIIa. EphB2 was cleaved by FVIIa concentrations in the subnanomolar range in a number of TF expressing cell types, indicating that the active cellular pool of TF was involved. FVIIa caused potentiation of cell repulsion by the EphB2 ligand ephrin-B1, demonstrating a novel proteolytical event to control Eph-mediated cell segregation. These results define Eph RTKs as novel proteolytical targets of TF/FVIIa and provide new insights into how TF/FVIIa regulates cellular functions independently of PAR2."xsd:string |
| http://purl.uniprot.org/citations/25281742 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m114.599332"xsd:string |
| http://purl.uniprot.org/citations/25281742 | http://purl.uniprot.org/core/author | "Bergquist J."xsd:string |
| http://purl.uniprot.org/citations/25281742 | http://purl.uniprot.org/core/author | "Eriksson O."xsd:string |
| http://purl.uniprot.org/citations/25281742 | http://purl.uniprot.org/core/author | "Siegbahn A."xsd:string |
| http://purl.uniprot.org/citations/25281742 | http://purl.uniprot.org/core/author | "Hornaeus K."xsd:string |
| http://purl.uniprot.org/citations/25281742 | http://purl.uniprot.org/core/author | "Mokhtari D."xsd:string |
| http://purl.uniprot.org/citations/25281742 | http://purl.uniprot.org/core/author | "Ramstrom M."xsd:string |
| http://purl.uniprot.org/citations/25281742 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25281742 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/25281742 | http://purl.uniprot.org/core/pages | "32379-32391"xsd:string |
| http://purl.uniprot.org/citations/25281742 | http://purl.uniprot.org/core/title | "The Eph tyrosine kinase receptors EphB2 and EphA2 are novel proteolytic substrates of tissue factor/coagulation factor VIIa."xsd:string |
| http://purl.uniprot.org/citations/25281742 | http://purl.uniprot.org/core/volume | "289"xsd:string |
| http://purl.uniprot.org/citations/25281742 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25281742 |
| http://purl.uniprot.org/citations/25281742 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25281742 |
| http://purl.uniprot.org/uniprot/#_A3RKG7-mappedCitation-25281742 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25281742 |
| http://purl.uniprot.org/uniprot/#_E2I6F7-mappedCitation-25281742 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25281742 |
| http://purl.uniprot.org/uniprot/#_B4DL04-mappedCitation-25281742 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25281742 |
| http://purl.uniprot.org/uniprot/#_B4DQP7-mappedCitation-25281742 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25281742 |
| http://purl.uniprot.org/uniprot/#_B4DSE0-mappedCitation-25281742 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25281742 |
| http://purl.uniprot.org/uniprot/#_A3RMR0-mappedCitation-25281742 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25281742 |
| http://purl.uniprot.org/uniprot/#_A3RMR1-mappedCitation-25281742 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25281742 |
| http://purl.uniprot.org/uniprot/#_B2R6M5-mappedCitation-25281742 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25281742 |
| http://purl.uniprot.org/uniprot/#_B4DPM2-mappedCitation-25281742 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25281742 |