| http://purl.uniprot.org/citations/25296750 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25296750 | http://www.w3.org/2000/01/rdf-schema#comment | "α-Actinin-4 (ACTN4), a key regulator of the actin cytoskeleton, is up-regulated in melanoma, though its role in melanoma remains speculative. We have discovered that in WM1158, a highly aggressive melanoma cell line, down-regulation of ACTN4 by shRNA induces a collagen I-dependent amoeboidal-to-mesenchymal transition. Re-expression of low levels of WT ACTN4 but not similar expression levels of ACTN1 successfully restores the amoeboidal morphology and limits collagen I gel compaction. A truncated ACTN4 mutant 1-890, which lacks the C-terminal tail, fails to rescue the amoeboidal morphology and to compact collagen I gel. Interestingly, in three-dimensional collagen I gels, ACTN4 KD cells are more polarized compared with cells in which scrambled shRNA is expressed. Surprisingly, ACTN4 KD cells migrate faster than the ones expressing the scrambled shRNA on a collagen I gel (two-dimensional) although these two cell lines migrate similarly on tissue culture. Most importantly, down-regulation of ACTN4 significantly reduced invasion of WM1158 cells into the three-dimensional collagen I gel, a representative of the dermis. Taken together, these findings suggest that ACTN4 plays an important role in maintaining the amoeboidal morphology of invasive melanoma and thus promoting dissemination through collagen-rich matrices."xsd:string |
| http://purl.uniprot.org/citations/25296750 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m114.579185"xsd:string |
| http://purl.uniprot.org/citations/25296750 | http://purl.uniprot.org/core/author | "Li S."xsd:string |
| http://purl.uniprot.org/citations/25296750 | http://purl.uniprot.org/core/author | "Shao H."xsd:string |
| http://purl.uniprot.org/citations/25296750 | http://purl.uniprot.org/core/author | "Watkins S.C."xsd:string |
| http://purl.uniprot.org/citations/25296750 | http://purl.uniprot.org/core/author | "Wells A."xsd:string |
| http://purl.uniprot.org/citations/25296750 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25296750 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/25296750 | http://purl.uniprot.org/core/pages | "32717-32728"xsd:string |
| http://purl.uniprot.org/citations/25296750 | http://purl.uniprot.org/core/title | "alpha-Actinin-4 is required for amoeboid-type invasiveness of melanoma cells."xsd:string |
| http://purl.uniprot.org/citations/25296750 | http://purl.uniprot.org/core/volume | "289"xsd:string |
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