| http://purl.uniprot.org/citations/2529854 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2529854 | http://www.w3.org/2000/01/rdf-schema#comment | "6-Phosphofructo-2-kinase (PFK-2) was purified from yeast and separated from fructose-2,6-biphosphatase (FBPase-2). The purification procedure involved polyethylene glycol fractionation followed by chromatography on DEAE-Sephacel. PFK-2 and FBPase-2 were copurified in these steps. Separation of the two enzymes resulted from Sephacryl S-300 Blue chromatography. Then, PFK-2 was chromatographed on CM-Sephadex and eluted with a gradient of KCl. Finally, PFK-2 was rechromatographed at CM-Sephadex and specifically eluted with fructose 6-phosphate. PFK-2 (specific activity 1.3 U/mg) was purified about 25,000-fold. The enzyme is inhibited by ATP which is particularly pronounced at low concentrations of magnesium and fructose 6-phosphate. Phosphoenolpyruvate and sn-glycerol 3-phosphate are inhibitors of the enzyme."xsd:string |
| http://purl.uniprot.org/citations/2529854 | http://purl.uniprot.org/core/author | "Kretschmer M."xsd:string |
| http://purl.uniprot.org/citations/2529854 | http://purl.uniprot.org/core/author | "Hofmann E."xsd:string |
| http://purl.uniprot.org/citations/2529854 | http://purl.uniprot.org/core/author | "Schellenberger W."xsd:string |
| http://purl.uniprot.org/citations/2529854 | http://purl.uniprot.org/core/author | "Bedri A."xsd:string |
| http://purl.uniprot.org/citations/2529854 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2529854 | http://purl.uniprot.org/core/name | "Biomed Biochim Acta"xsd:string |
| http://purl.uniprot.org/citations/2529854 | http://purl.uniprot.org/core/pages | "403-411"xsd:string |
| http://purl.uniprot.org/citations/2529854 | http://purl.uniprot.org/core/title | "Kinetics of 6-phosphofructo-2-kinase from Saccharomyces cerevisiae: inhibition of the enzyme by ATP."xsd:string |
| http://purl.uniprot.org/citations/2529854 | http://purl.uniprot.org/core/volume | "48"xsd:string |
| http://purl.uniprot.org/citations/2529854 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2529854 |
| http://purl.uniprot.org/citations/2529854 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2529854 |
| http://purl.uniprot.org/uniprot/#_P40433-mappedCitation-2529854 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/2529854 |
| http://purl.uniprot.org/uniprot/#_Q12471-mappedCitation-2529854 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/2529854 |
| http://purl.uniprot.org/uniprot/P40433 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/2529854 |
| http://purl.uniprot.org/uniprot/Q12471 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/2529854 |