| http://purl.uniprot.org/citations/25324544 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25324544 | http://www.w3.org/2000/01/rdf-schema#comment | "Retinoid X receptor (RXRα) is activated by 9-cis-retinoic acid (9cRA) and regulates transcription as a homodimer or as a heterodimer with other nuclear receptors. We have previously demonstrated that β-apo-13-carotenone, an eccentric cleavage product of β-carotene, antagonizes the activation of RXRα by 9cRA in mammalian cells overexpressing this receptor. However, the molecular mechanism of β-apo-13-carotenone's modulation on the transcriptional activity of RXRα is not understood and is the subject of this report. We performed transactivation assays using full-length RXRα and reporter gene constructs (RXRE-Luc) transfected into COS-7 cells, and luciferase activity was examined. β-Apo-13-carotenone was compared with the RXRα antagonist UVI3003. The results showed that both β-apo-13-carotenone and UVI3003 shifted the dose-dependent RXRα activation by 9cRA. In contrast, the results of assays using a hybrid Gal4-DBD:RXRαLBD receptor reporter cell assay that detects 9cRA-induced coactivator binding to the ligand binding domain demonstrated that UVI3003 significantly inhibited 9cRA-induced coactivator binding to RXRαLBD, but β-apo-13-carotenone did not. However, both β-apo-13-carotenone and UVI3003 inhibited 9-cRA induction of caspase 9 gene expression in the mammary carcinoma cell line MCF-7. To resolve this apparent contradiction, we investigated the effect of β-apo-13-carotenone on the oligomeric state of purified recombinant RXRαLBD. β-Apo-13-carotenone induces tetramerization of the RXRαLBD, although UVI3003 had no effect on the oligomeric state. These observations suggest that β-apo-13-carotenone regulates RXRα transcriptional activity by inducing the formation of the "transcriptionally silent" RXRα tetramer."xsd:string |
| http://purl.uniprot.org/citations/25324544 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m114.610501"xsd:string |
| http://purl.uniprot.org/citations/25324544 | http://purl.uniprot.org/core/author | "Sun J."xsd:string |
| http://purl.uniprot.org/citations/25324544 | http://purl.uniprot.org/core/author | "Curley R.W. Jr."xsd:string |
| http://purl.uniprot.org/citations/25324544 | http://purl.uniprot.org/core/author | "Harrison E.H."xsd:string |
| http://purl.uniprot.org/citations/25324544 | http://purl.uniprot.org/core/author | "Narayanasamy S."xsd:string |
| http://purl.uniprot.org/citations/25324544 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25324544 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/25324544 | http://purl.uniprot.org/core/pages | "33118-33124"xsd:string |
| http://purl.uniprot.org/citations/25324544 | http://purl.uniprot.org/core/title | "beta-Apo-13-carotenone regulates retinoid X receptor transcriptional activity through tetramerization of the receptor."xsd:string |
| http://purl.uniprot.org/citations/25324544 | http://purl.uniprot.org/core/volume | "289"xsd:string |
| http://purl.uniprot.org/citations/25324544 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25324544 |
| http://purl.uniprot.org/citations/25324544 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25324544 |
| http://purl.uniprot.org/uniprot/#_A0A5F8MP91-mappedCitation-25324544 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25324544 |
| http://purl.uniprot.org/uniprot/#_A0A5F8MQ23-mappedCitation-25324544 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25324544 |
| http://purl.uniprot.org/uniprot/#_A2AJP1-mappedCitation-25324544 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25324544 |
| http://purl.uniprot.org/uniprot/#_A2AJP2-mappedCitation-25324544 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25324544 |
| http://purl.uniprot.org/uniprot/#_P28700-mappedCitation-25324544 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25324544 |
| http://purl.uniprot.org/uniprot/#_Q3UMU4-mappedCitation-25324544 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25324544 |
| http://purl.uniprot.org/uniprot/#_Q6LC96-mappedCitation-25324544 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25324544 |
| http://purl.uniprot.org/uniprot/A0A5F8MP91 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25324544 |
| http://purl.uniprot.org/uniprot/Q3UMU4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25324544 |
| http://purl.uniprot.org/uniprot/A2AJP1 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25324544 |
| http://purl.uniprot.org/uniprot/P28700 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25324544 |