| http://purl.uniprot.org/citations/25336627 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25336627 | http://www.w3.org/2000/01/rdf-schema#comment | "O-glycosylation of podoplanin (PDPN) on lymphatic endothelial cells is critical for the separation of blood and lymphatic systems by interacting with platelet C-type lectin-like receptor 2 during development. However, how O-glycosylation controls endothelial PDPN function and expression remains unclear. In this study, we report that core 1 O-glycan-deficient or desialylated PDPN was highly susceptible to proteolytic degradation by various proteases, including metalloproteinases (MMP)-2/9. We found that the lymph contained activated MMP-2/9 and incubation of the lymph reduced surface levels of PDPN on core 1 O-glycan-deficient endothelial cells, but not on wild-type ECs. The lymph from mice with sepsis induced by cecal ligation and puncture, which contained bacteria-derived sialidase, reduced PDPN levels on wild-type ECs. The MMP inhibitor, GM6001, rescued these reductions. Additionally, GM6001 treatment rescued the reduction of PDPN level on lymphatic endothelial cells in mice lacking endothelial core 1 O-glycan or cecal ligation and puncture-treated mice. Furthermore, core 1 O-glycan-deficient or desialylated PDPN impaired platelet interaction under physiological flow. These data indicate that sialylated O-glycans of PDPN are essential for platelet adhesion and prevent PDPN from proteolytic degradation primarily mediated by MMPs in the lymph."xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood-2014-04-572107"xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/author | "Cai X."xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/author | "Chen H."xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/author | "Pan Y."xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/author | "Xia L."xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/author | "Fu J."xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/author | "Ruan C."xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/author | "Herzog B."xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/author | "West C."xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/author | "McEver R.P."xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/author | "McDaniel J.M."xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/author | "Dai K."xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/author | "Yago T."xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/author | "Mehta-D'Souza P."xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/pages | "3656-3665"xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/title | "Podoplanin requires sialylated O-glycans for stable expression on lymphatic endothelial cells and for interaction with platelets."xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://purl.uniprot.org/core/volume | "124"xsd:string |
| http://purl.uniprot.org/citations/25336627 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25336627 |
| http://purl.uniprot.org/citations/25336627 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25336627 |
| http://purl.uniprot.org/uniprot/#_A0T1G3-mappedCitation-25336627 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25336627 |
| http://purl.uniprot.org/uniprot/#_A8Y5F6-mappedCitation-25336627 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25336627 |