| http://purl.uniprot.org/citations/25340394 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25340394 | http://www.w3.org/2000/01/rdf-schema#comment | "Glycolytic potential (GP) in skeletal muscle is economically important in the pig industry because of its effect on pork processing yield. We have previously mapped a major quantitative trait loci (QTL) for GP on chromosome 3 in a White Duroc × Erhualian F2 intercross. We herein performed a systems genetic analysis to identify the causal variant underlying the phenotype QTL (pQTL). We first conducted genome-wide association analyses in the F2 intercross and an F19 Sutai pig population. The QTL was then refined to an 180-kb interval based on the 2-LOD drop method. We then performed expression QTL (eQTL) mapping using muscle transcriptome data from 497 F2 animals. Within the QTL interval, only one gene (PHKG1) has a cis-eQTL that was colocolizated with pQTL peaked at the same SNP. The PHKG1 gene encodes a catalytic subunit of the phosphorylase kinase (PhK), which functions in the cascade activation of glycogen breakdown. Deep sequencing of PHKG1 revealed a point mutation (C>A) in a splice acceptor site of intron 9, resulting in a 32-bp deletion in the open reading frame and generating a premature stop codon. The aberrant transcript induces nonsense-mediated decay, leading to lower protein level and weaker enzymatic activity in affected animals. The mutation causes an increase of 43% in GP and a decrease of>20% in water-holding capacity of pork. These effects were consistent across the F2 and Sutai populations, as well as Duroc × (Landrace × Yorkshire) hybrid pigs. The unfavorable allele exists predominantly in Duroc-derived pigs. The findings provide new insights into understanding risk factors affecting glucose metabolism, and would greatly contribute to the genetic improvement of meat quality in Duroc related pigs."xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pgen.1004710"xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/author | "Huang L."xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/author | "Guo Y."xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/author | "Liu X."xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/author | "Ma J."xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/author | "Ma H."xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/author | "Ren J."xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/author | "Yang J."xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/author | "Zhou L."xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/author | "Zhang Z."xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/author | "Zhang H."xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/author | "Xie X."xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/author | "Xing Y."xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/author | "Yang B."xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/name | "PLoS Genet"xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/pages | "e1004710"xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/title | "A splice mutation in the PHKG1 gene causes high glycogen content and low meat quality in pig skeletal muscle."xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/25340394 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25340394 |
| http://purl.uniprot.org/citations/25340394 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25340394 |
| http://purl.uniprot.org/uniprot/P07934#attribution-DFD889A45D178B69EEC353507B4D65AE | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/25340394 |
| http://purl.uniprot.org/uniprot/#_D3Z1B5-mappedCitation-25340394 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25340394 |