| http://purl.uniprot.org/citations/25354530 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25354530 | http://www.w3.org/2000/01/rdf-schema#comment | "Protein scaffolds can provide a promising alternative to antibodies for various biomedical and biotechnological applications, including therapeutics. Here we describe the design and development of the Alphabody, a protein scaffold featuring a single-chain antiparallel triple-helix coiled-coil fold. We report affinity-matured Alphabodies with favourable physicochemical properties that can specifically neutralize human interleukin (IL)-23, a pivotal therapeutic target in autoimmune inflammatory diseases such as psoriasis and multiple sclerosis. The crystal structure of human IL-23 in complex with an affinity-matured Alphabody reveals how the variable interhelical groove of the scaffold uniquely targets a large epitope on the p19 subunit of IL-23 to harness fully the hydrophobic and hydrogen-bonding potential of tryptophan and tyrosine residues contributed by p19 and the Alphabody, respectively. Thus, Alphabodies are suitable for targeting protein-protein interfaces of therapeutic importance and can be tailored to interrogate desired design and binding-mode principles via efficient selection and affinity-maturation strategies."xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.org/dc/terms/identifier | "doi:10.1038/ncomms6237"xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/author | "Devreese B."xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/author | "Hettmann T."xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/author | "Wen Y."xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/author | "Savvides S.N."xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/author | "Bloch Y."xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/author | "Verstraete K."xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/author | "Loverix S."xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/author | "Vandenbroucke K."xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/author | "Lasters I."xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/author | "Henderikx P."xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/author | "Desmet J."xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/author | "Deroo S."xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/author | "Somers K."xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/author | "Lorent E."xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/name | "Nat Commun"xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/pages | "5237"xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/title | "Structural basis of IL-23 antagonism by an Alphabody protein scaffold."xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://purl.uniprot.org/core/volume | "5"xsd:string |
| http://purl.uniprot.org/citations/25354530 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25354530 |
| http://purl.uniprot.org/citations/25354530 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25354530 |
| http://purl.uniprot.org/uniprot/#_Q9NPF7-mappedCitation-25354530 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25354530 |