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http://purl.uniprot.org/citations/25368324http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/25368324http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/25368324http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Citation
http://purl.uniprot.org/citations/25368324http://www.w3.org/2000/01/rdf-schema#comment"Surface glycan switching is often observed when micro-organisms transition between different biotic and abiotic niches, including biofilms, although the advantages of this switching to the organism are not well understood. Bacillus cereus grown in a biofilm-inducing medium has been shown to synthesize an unusual cell wall polysaccharide composed of the repeating subunit →6)Gal(α1-2)(2-R-hydroxyglutar-5-ylamido)Fuc2NAc4N(α1-6)GlcNAc(β1→, where galactose is linked to the hydroxyglutarate moiety of FucNAc-4-amido-(2)-hydroxyglutarate. The molecular mechanism involved in attaching 2-hydroxyglutarate to 4-amino-FucNAc has not been determined. Here, we show two genes in B. cereus ATCC 14579 encoding enzymes involved in the synthesis of UDP-FucNAc-4-amido-(2)-oxoglutarate (UDP-Yelosamine), a modified UDP-sugar not previously reported to exist. Using mass spectrometry and real time NMR spectroscopy, we show that Bc5273 encodes a C4″-aminotransferase (herein referred to as Pat) that, in the presence of pyridoxal phosphate, transfers the primary amino group of l-Glu to C-4″ of UDP-4-keto-6-deoxy-d-GlcNAc to form UDP-4-amino-FucNAc and 2-oxoglutarate. Pat also converts 4-keto-xylose, 4-keto-glucose, and 4-keto-2-acetamido-altrose to their corresponding UDP-4-amino-sugars. Bc5272 encodes a carboxylate-amine ligase (herein referred as Pyl) that, in the presence of ATP and Mg(II), adds 2-oxoglutarate to the 4-amino moiety of UDP-4-amino-FucNAc to form UDP-Yelosamine and ADP. Pyl is also able to ligate 2-oxoglutarate to other 4-amino-sugar derivatives to form UDP-Yelose, UDP-Solosamine, and UDP-Aravonose. Characterizing the metabolic pathways involved in the formation of modified nucleotide sugars provides a basis for understanding some of the mechanisms used by bacteria to modify or alter their cell surface polysaccharides in response to changing growth and environmental challenges."xsd:string
http://purl.uniprot.org/citations/25368324http://purl.org/dc/terms/identifier"doi:10.1074/jbc.m114.614917"xsd:string
http://purl.uniprot.org/citations/25368324http://purl.org/dc/terms/identifier"doi:10.1074/jbc.m114.614917"xsd:string
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/author"Li Z."xsd:string
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/author"Li Z."xsd:string
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/author"Hwang S."xsd:string
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/author"Hwang S."xsd:string
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/author"Bar-Peled M."xsd:string
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/author"Bar-Peled M."xsd:string
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/author"Ericson J."xsd:string
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/author"Ericson J."xsd:string
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/author"Aronov A."xsd:string
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/author"Aronov A."xsd:string
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/author"Bar-Peled Y."xsd:string
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/author"Bar-Peled Y."xsd:string
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/date"2014"xsd:gYear
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/date"2014"xsd:gYear
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/name"J Biol Chem"xsd:string
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/pages"35620-35632"xsd:string
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/title"The biosynthesis of UDP-D-FucNAc-4N-(2)-oxoglutarate (UDP-Yelosamine) in Bacillus cereus ATCC 14579: Pat and Pyl, an aminotransferase and an ATP-dependent grasp protein that ligates 2-oxoglutarate to UDP-4-amino-sugars."xsd:string
http://purl.uniprot.org/citations/25368324http://purl.uniprot.org/core/title"The biosynthesis of UDP-d-FucNAc-4N-(2)-oxoglutarate (UDP-Yelosamine) in Bacillus cereus ATCC 14579: Pat and Pyl, an aminotransferase and an ATP-dependent Grasp protein that ligates 2-oxoglutarate to UDP-4-amino-sugars."xsd:string
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