| http://purl.uniprot.org/citations/2538439 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2538439 | http://www.w3.org/2000/01/rdf-schema#comment | "Platelet-derived growth factor (PDGF) is known to inhibit collagen-induced platelet aggregation. Collagen-induced binding of 125I-PDGF to human washed platelets was therefore investigated. It was found 1) to be time-dependent, reaching a plateau at 20 degrees C after 30 min, 2) collagen concentration-dependent, 3) specifically inhibited by unlabeled PDGF, and 4) saturable. Scatchard plot analysis showed a single class of sites with 3000 +/-450 molecules bound/cell and an apparent KD of 1.2 +/-0.2 10(-8) M. The effects of PDGF on collagen-induced phosphoinositide breakdown and protein phosphorylation were also investigated. At 50 ng/ml PDGF, a concentration which completely inhibited collagen-induced aggregation, the breakdown of [32P]phosphatidylinositol 4,5-biphosphate (PIP2) and [32P]phosphatidylinositol 4-phosphate (PIP) was observed, but the subsequent replenishment of [32P]PIP2 was inhibited. The same PDGF concentration totally inhibited collagen-induced phosphatidic acid formation. PDGF also completely prevented phosphorylation of P43 and P20, as a result of protein kinase C activation consecutive to phosphoinositide metabolism. These results suggest that (i) a specific PDGF receptor can be induced by collagen, and (ii) PDGF can effect the early events of collagen-induced platelet activation by inhibiting PIP2 resynthesis and P43 and P20 phosphorylation. It is concluded that PDGF might be involved in a negative feed-back control of platelet activation."xsd:string |
| http://purl.uniprot.org/citations/2538439 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)83746-2"xsd:string |
| http://purl.uniprot.org/citations/2538439 | http://purl.uniprot.org/core/author | "Rendu F."xsd:string |
| http://purl.uniprot.org/citations/2538439 | http://purl.uniprot.org/core/author | "Wasteson A."xsd:string |
| http://purl.uniprot.org/citations/2538439 | http://purl.uniprot.org/core/author | "Bryckaert M.C."xsd:string |
| http://purl.uniprot.org/citations/2538439 | http://purl.uniprot.org/core/author | "Tobelem G."xsd:string |
| http://purl.uniprot.org/citations/2538439 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2538439 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/2538439 | http://purl.uniprot.org/core/pages | "4336-4341"xsd:string |
| http://purl.uniprot.org/citations/2538439 | http://purl.uniprot.org/core/title | "Collagen-induced binding to human platelets of platelet-derived growth factor leading to inhibition of P43 and P20 phosphorylation."xsd:string |
| http://purl.uniprot.org/citations/2538439 | http://purl.uniprot.org/core/volume | "264"xsd:string |
| http://purl.uniprot.org/citations/2538439 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2538439 |
| http://purl.uniprot.org/citations/2538439 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2538439 |
| http://purl.uniprot.org/uniprot/P01127#attribution-D13762C7573D6168AA4AAB68E43897F7 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/2538439 |
| http://purl.uniprot.org/uniprot/P04085#attribution-D13762C7573D6168AA4AAB68E43897F7 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/2538439 |