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http://purl.uniprot.org/citations/25410898http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/25410898http://www.w3.org/2000/01/rdf-schema#comment"

Background

The rostral ventromedial medulla (RVM) is a key brainstem structure that conveys powerful descending influence of the central pain-modulating system on spinal pain transmission and processing. Serotonergic (5-HT) neurons are a major component in the heterogeneous populations of RVM neurons and in the descending pathways from RVM. However, the descending influence of RVM 5-HT neurons on pain behaviors remains unclear.

Results

In this study using optogenetic stimulation in tryptophan hydroxylase 2 (TPH2)-Channelrhodopsin 2 (ChR2) transgenic mice, we determined the behavioral effects of selective activation of RVM 5-HT neurons on mechanical and thermal pain behaviors in vivo. We found that ChR2-EYFP-positive neurons strongly co-localized with TPH2-positive (5-HT) neurons in RVM. Optogenetic stimulation significantly increased c-fos expression in 5-HT cells in the RVM of TPH2-ChR2 mice, but not in wild type mice. Behaviorally, the optogenetic stimulation decreased both mechanical and thermal pain threshold in an intensity-dependent manner, with repeated stimulation producing sensitized pain behavior for up to two weeks.

Conclusions

These results suggest that selective activation of RVM 5-HT neurons exerts a predominant effect of pain facilitation under control conditions."xsd:string
http://purl.uniprot.org/citations/25410898http://purl.org/dc/terms/identifier"doi:10.1186/1744-8069-10-70"xsd:string
http://purl.uniprot.org/citations/25410898http://purl.uniprot.org/core/author"Wang W."xsd:string
http://purl.uniprot.org/citations/25410898http://purl.uniprot.org/core/author"Pan Z.Z."xsd:string
http://purl.uniprot.org/citations/25410898http://purl.uniprot.org/core/author"Hou Y.Y."xsd:string
http://purl.uniprot.org/citations/25410898http://purl.uniprot.org/core/author"Cai Y.Q."xsd:string
http://purl.uniprot.org/citations/25410898http://purl.uniprot.org/core/date"2014"xsd:gYear
http://purl.uniprot.org/citations/25410898http://purl.uniprot.org/core/name"Mol Pain"xsd:string
http://purl.uniprot.org/citations/25410898http://purl.uniprot.org/core/pages"70"xsd:string
http://purl.uniprot.org/citations/25410898http://purl.uniprot.org/core/title"Optogenetic activation of brainstem serotonergic neurons induces persistent pain sensitization."xsd:string
http://purl.uniprot.org/citations/25410898http://purl.uniprot.org/core/volume"10"xsd:string
http://purl.uniprot.org/citations/25410898http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/25410898
http://purl.uniprot.org/citations/25410898http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/25410898
http://purl.uniprot.org/uniprot/#_Q8CGV2-mappedCitation-25410898http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/25410898
http://purl.uniprot.org/uniprot/Q8CGV2http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/25410898