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http://purl.uniprot.org/citations/25439783http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
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Objectives

Rapamycin inhibits products of molecular pathways in esophageal squamous cell carcinoma and limits tumor cell growth by targeting 4E-BP1- and eIF4E-dependent gene translation. In this study, we investigate the influence of 4E-BP1-to-eIF4E ratio on rapamycin response in esophageal squamous cell carcinoma cells, and the underlying mechanism is discussed.

Methods

The response to rapamycin treatment was examined in 6 esophageal cancer cell lines. Adjustment of the 4E-BP1/eIF4E ratio was carried out by knockdown or overexpression of 4E-BP1 and eIF4E. The relationship between Egr-1 and 4E-BP1 expression in esophageal cancer cells was also studied.

Results

The 4E-BP1/eIF4E ratio was adjusted to evaluate the response to rapamycin treatment in TE1 and TE2 esophageal cancer cells. TE2 cells are sensitized to rapamycin treatment after overexpression of 4E-BP1 or knockdown of eIF4E; TE1 cells become resistant to rapamycin after knockdown of 4E-BP1 or overexpression of eIF4E. These data suggest that the 4E-BP1/eIF4E ratio is a determinant for the response of TE1 and TE2 cells to rapamycin treatment. Egr-1 expression was higher in TE2 cells compared with other esophageal cancer cell lines, and its knockdown increased 4E-BP1 expression in TE2 cells, which became sensitive to rapamycin treatment.

Conclusions

The 4E-BP1/eIF4E ratio is a determinant of the response of rapamycin treatment in esophageal cancer cells. Egr-1 can reduce 4E-BP1 gene expression and render esophageal squamous cell carcinoma cells resistant to rapamycin with a relatively low 4E-BP1/eIF4E ratio. Thus, the 4E-BP1/eIF4E ratio may represent a therapeutic index for the prediction of clinical outcome of rapamycin treatment in patients with esophageal squamous cell carcinoma."xsd:string
http://purl.uniprot.org/citations/25439783http://purl.org/dc/terms/identifier"doi:10.1016/j.jtcvs.2014.09.047"xsd:string
http://purl.uniprot.org/citations/25439783http://purl.uniprot.org/core/author"Liu C.C."xsd:string
http://purl.uniprot.org/citations/25439783http://purl.uniprot.org/core/author"Kuo T.T."xsd:string
http://purl.uniprot.org/citations/25439783http://purl.uniprot.org/core/author"Lin M.H."xsd:string
http://purl.uniprot.org/citations/25439783http://purl.uniprot.org/core/author"Jang Y.H."xsd:string
http://purl.uniprot.org/citations/25439783http://purl.uniprot.org/core/author"Cheng T.H."xsd:string
http://purl.uniprot.org/citations/25439783http://purl.uniprot.org/core/author"Hsu H.S."xsd:string
http://purl.uniprot.org/citations/25439783http://purl.uniprot.org/core/date"2015"xsd:gYear
http://purl.uniprot.org/citations/25439783http://purl.uniprot.org/core/name"J Thorac Cardiovasc Surg"xsd:string
http://purl.uniprot.org/citations/25439783http://purl.uniprot.org/core/pages"378-385"xsd:string
http://purl.uniprot.org/citations/25439783http://purl.uniprot.org/core/title"The 4E-BP1/eIF4E ratio is a determinant for rapamycin response in esophageal cancer cells."xsd:string
http://purl.uniprot.org/citations/25439783http://purl.uniprot.org/core/volume"149"xsd:string
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