| http://purl.uniprot.org/citations/25468995 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25468995 | http://www.w3.org/2000/01/rdf-schema#comment | "In the thymus, immature T cells differentiate from common precursors to become T cells expressing either the αβ or γδ T cell receptor (TCR) complex. The CD3ε subunit of the TCR complex is thought to transduce ligand-induced conformational changes in the TCR by recruiting the cytosolic adaptor protein Nck. To investigate the role of conformational changes in the TCR in T cell development, we generated mice with a germline mutation (C80G) in the extracellular domain of CD3ε, which prevents the outside-in transmission of conformational changes in the TCR. The development of αβ T cells in the C80G mice was blocked at an early stage that depends on signaling by a precursor form of the TCR. In contrast, the C80G mutation did not impair the development of some subsets of γδ T cells, including Vγ1.1(+) cells; however, development of other γδ T cell subsets was blocked. A similar phenotype was observed in mice with a mutation in the cytoplasmic proline-rich sequence (PRS) of CD3ε, the binding site for Nck. In a genetic complementation test, the PRS CD3ε mutant failed to rescue the wild-type phenotype when expressed in heterozygosity with the C80G mutant. These data suggest that Nck may function as an effector of TCR conformational changes during T cell development. Additional experiments showed differential effects of the C80G mutation on the activation of TCR-dependent signaling pathways, which suggests that there are pathways that are either dependent on or independent of the transmission of conformational change in the receptor."xsd:string |
| http://purl.uniprot.org/citations/25468995 | http://purl.org/dc/terms/identifier | "doi:10.1126/scisignal.2005650"xsd:string |
| http://purl.uniprot.org/citations/25468995 | http://purl.uniprot.org/core/author | "Alarcon B."xsd:string |
| http://purl.uniprot.org/citations/25468995 | http://purl.uniprot.org/core/author | "Blanco R."xsd:string |
| http://purl.uniprot.org/citations/25468995 | http://purl.uniprot.org/core/author | "Borroto A."xsd:string |
| http://purl.uniprot.org/citations/25468995 | http://purl.uniprot.org/core/author | "Pereira P."xsd:string |
| http://purl.uniprot.org/citations/25468995 | http://purl.uniprot.org/core/author | "Schamel W."xsd:string |
| http://purl.uniprot.org/citations/25468995 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25468995 | http://purl.uniprot.org/core/name | "Sci Signal"xsd:string |
| http://purl.uniprot.org/citations/25468995 | http://purl.uniprot.org/core/pages | "ra115"xsd:string |
| http://purl.uniprot.org/citations/25468995 | http://purl.uniprot.org/core/title | "Conformational changes in the T cell receptor differentially determine T cell subset development in mice."xsd:string |
| http://purl.uniprot.org/citations/25468995 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/25468995 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25468995 |
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| http://purl.uniprot.org/uniprot/#_A0A087WSB1-mappedCitation-25468995 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25468995 |
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| http://purl.uniprot.org/uniprot/#_B0LAX5-mappedCitation-25468995 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25468995 |
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