| http://purl.uniprot.org/citations/25516977 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25516977 | http://www.w3.org/2000/01/rdf-schema#comment | "During skeletal muscle development, nuclei move dynamically through myotubes in a microtubule-dependent manner, driven by the microtubule motor protein kinesin-1. Loss of kinesin-1 leads to improperly positioned nuclei in culture and in vivo. Two models have been proposed to explain how kinesin-1 functions to move nuclei in myotubes. In the cargo model, kinesin-1 acts directly from the surface of the nucleus, whereas in an alternative model, kinesin-1 moves nuclei indirectly by sliding anti-parallel microtubules. Here, we test the hypothesis that an ensemble of Kif5B motors acts from the nuclear envelope to distribute nuclei throughout the length of syncytial myotubes. First, using an inducible dimerization system, we show that controlled recruitment of truncated, constitutively active kinesin-1 motors to the nuclear envelope is sufficient to prevent the nuclear aggregation resulting from depletion of endogenous kinesin-1. Second, we identify a conserved kinesin light chain (KLC)-binding motif in the nuclear envelope proteins nesprin-1 and nesprin-2, and show that recruitment of the motor complex to the nucleus via this LEWD motif is essential for nuclear distribution. Together, our findings demonstrate that the nucleus is a kinesin-1 cargo in myotubes and that nesprins function as nuclear cargo adaptors. The importance of achieving and maintaining proper nuclear position is not restricted to muscle fibers, suggesting that the nesprin-dependent recruitment of kinesin-1 to the nuclear envelope through the interaction of a conserved LEWD motif with kinesin light chain might be a general mechanism for cell-type-specific nuclear positioning during development."xsd:string |
| http://purl.uniprot.org/citations/25516977 | http://purl.org/dc/terms/identifier | "doi:10.1242/dev.114769"xsd:string |
| http://purl.uniprot.org/citations/25516977 | http://purl.uniprot.org/core/author | "Holzbaur E.L."xsd:string |
| http://purl.uniprot.org/citations/25516977 | http://purl.uniprot.org/core/author | "Wilson M.H."xsd:string |
| http://purl.uniprot.org/citations/25516977 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25516977 | http://purl.uniprot.org/core/name | "Development"xsd:string |
| http://purl.uniprot.org/citations/25516977 | http://purl.uniprot.org/core/pages | "218-228"xsd:string |
| http://purl.uniprot.org/citations/25516977 | http://purl.uniprot.org/core/title | "Nesprins anchor kinesin-1 motors to the nucleus to drive nuclear distribution in muscle cells."xsd:string |
| http://purl.uniprot.org/citations/25516977 | http://purl.uniprot.org/core/volume | "142"xsd:string |
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