Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/25517360http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/25517360http://www.w3.org/2000/01/rdf-schema#comment"

Background

Differences in 5-hydroxymethylcytosine, 5hmC, distributions may complicate previous observations of abnormal cytosine methylation statuses that are used for the identification of new tumor suppressor gene candidates that are relevant to human hepatocarcinogenesis. The simultaneous detection of 5-methylcytosine and 5-hydroxymethylcytosine is likely to stimulate the discovery of aberrantly methylated genes with increased accuracy in human hepatocellular carcinoma.

Results

Here, we performed ultra-performance liquid chromatography/tandem mass spectrometry and single-base high-throughput sequencing, Hydroxymethylation and Methylation Sensitive Tag sequencing, HMST-seq, to synchronously measure these two modifications in human hepatocellular carcinoma samples. After identification of differentially methylated and hydroxymethylated genes in human hepatocellular carcinoma, we integrate DNA copy-number alterations, as determined using array-based comparative genomic hybridization data, with gene expression to identify genes that are potentially silenced by promoter hypermethylation.

Conclusions

We report a high enrichment of genes with epigenetic aberrations in cancer signaling pathways. Six genes were selected as tumor suppressor gene candidates, among which, ECM1, ATF5 and EOMES are confirmed via siRNA experiments to have potential anti-cancer functions."xsd:string
http://purl.uniprot.org/citations/25517360http://purl.org/dc/terms/identifier"doi:10.1186/s13059-014-0533-9"xsd:string
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/author"Huang J."xsd:string
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/author"Gao F."xsd:string
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/author"Liu X."xsd:string
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/author"Liu W."xsd:string
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/author"Lin Z."xsd:string
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/author"Luo H."xsd:string
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/author"Ou Y."xsd:string
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/author"Zhang X."xsd:string
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/author"Wang J."xsd:string
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/author"Xia Y."xsd:string
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/author"Zhou B."xsd:string
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/author"Wen B."xsd:string
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/author"Zhou B.'"xsd:string
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/date"2014"xsd:gYear
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/name"Genome Biol"xsd:string
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/pages"533"xsd:string
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/title"Integrated analyses of DNA methylation and hydroxymethylation reveal tumor suppressive roles of ECM1, ATF5, and EOMES in human hepatocellular carcinoma."xsd:string
http://purl.uniprot.org/citations/25517360http://purl.uniprot.org/core/volume"15"xsd:string
http://purl.uniprot.org/citations/25517360http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/25517360
http://purl.uniprot.org/citations/25517360http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/25517360
http://purl.uniprot.org/uniprot/#_A0A140VJI7-mappedCitation-25517360http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/25517360
http://purl.uniprot.org/uniprot/#_A0A510GFN1-mappedCitation-25517360http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/25517360
▶