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http://purl.uniprot.org/citations/25523922http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
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Background

Curcumin (CUR), an active polyphenol derived from the spice turmeric, has been traditionally used for centuries in ancient Indian medicine to treat a number of diseases. The physiological effects of CUR have been shown to be diverse; however, the target molecules and pathways that CUR affects have yet to be fully described.

Results

Here, we demonstrate for the first time that the budding yeast mitogen-activated protein kinase (MAPK) Hog1 is essential for the response to CUR. Moreover, CUR-induced Hog1 phosphorylation was rescued by supplementation of iron to the growth medium. Hog1 was rapidly phosphorylated upon CUR treatment, but unlike the response to hyperosmotic shock (0.8 M NaCl), it remains activated for an extended period of time. A detailed analysis of HOG pathway mutants revealed that Pbs2p, Ptc2p, and Ssk2p are required for optimal CUR-induced Hog1 phosphorylation. We also observed a Hog1 dependent transcriptional response to CUR treatment that involved the up-regulation of glycerol-3-phosphate dehydrogenase 1 (GPD1), a factor that is essential for the hyperosmotic stress response.

Conclusions

Our present finding revealed the role of Hog1 MAPK in regulation of CUR-induced transcriptional response. We anticipate that our finding will enhance the understanding on the molecular mode of action of CUR on S. cerevisiae."xsd:string
http://purl.uniprot.org/citations/25523922http://purl.org/dc/terms/identifier"doi:10.1186/s12866-014-0317-0"xsd:string
http://purl.uniprot.org/citations/25523922http://purl.uniprot.org/core/author"Singh V."xsd:string
http://purl.uniprot.org/citations/25523922http://purl.uniprot.org/core/author"Tomar R.S."xsd:string
http://purl.uniprot.org/citations/25523922http://purl.uniprot.org/core/author"Baranwal S."xsd:string
http://purl.uniprot.org/citations/25523922http://purl.uniprot.org/core/author"Azad G.K."xsd:string
http://purl.uniprot.org/citations/25523922http://purl.uniprot.org/core/author"Thakare M.J."xsd:string
http://purl.uniprot.org/citations/25523922http://purl.uniprot.org/core/date"2014"xsd:gYear
http://purl.uniprot.org/citations/25523922http://purl.uniprot.org/core/name"BMC Microbiol"xsd:string
http://purl.uniprot.org/citations/25523922http://purl.uniprot.org/core/pages"317"xsd:string
http://purl.uniprot.org/citations/25523922http://purl.uniprot.org/core/title"Mitogen-activated protein kinase Hog1 is activated in response to curcumin exposure in the budding yeast Saccharomyces cerevisiae."xsd:string
http://purl.uniprot.org/citations/25523922http://purl.uniprot.org/core/volume"14"xsd:string
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