| http://purl.uniprot.org/citations/25543119 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25543119 | http://www.w3.org/2000/01/rdf-schema#comment | "Reticulons (RTNs) constitute a family of endoplasmic reticulum (ER)-associated proteins with a reticular distribution. Recently, evidence has shown that they exert a cancer-specific proapoptotic function via interaction or modulation of specific proteins. Such evidence is particularly associated with the RTN1-C family members. In order to explore proteins that interact with RTN1-C, the yeast two-hybrid system and regular molecular biological techniques were used to screen the human fetal brain cDNA library. As a result, seven RTN1-C interacting proteins including Homo sapiens mesencephalic astrocyte-derived neurotrophic factor (MANF) were obtained. The interactions between RTN1-C and its interacting proteins were confirmed by β-galactosidase assay and growth test in selective media. Moreover, the MANF/RTN1-C interaction was verified in vitro by glutathione S-transferase pull-down assay and in vivo by immunoprecipitation assay. By immunofluorescence assay, it was found that MANF co-localized with RTN1-C in the ER. Knockdown of RTN1-C reduced the localization of MANF in the ER. These results provide clues to further explore the function of RTN1-C and MANF in neurodegenerative diseases and cancer."xsd:string |
| http://purl.uniprot.org/citations/25543119 | http://purl.org/dc/terms/identifier | "doi:10.1093/abbs/gmu125"xsd:string |
| http://purl.uniprot.org/citations/25543119 | http://purl.uniprot.org/core/author | "Chen L."xsd:string |
| http://purl.uniprot.org/citations/25543119 | http://purl.uniprot.org/core/author | "Du J."xsd:string |
| http://purl.uniprot.org/citations/25543119 | http://purl.uniprot.org/core/author | "Shen Y."xsd:string |
| http://purl.uniprot.org/citations/25543119 | http://purl.uniprot.org/core/author | "Wan L."xsd:string |
| http://purl.uniprot.org/citations/25543119 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25543119 | http://purl.uniprot.org/core/name | "Acta Biochim Biophys Sin (Shanghai)"xsd:string |
| http://purl.uniprot.org/citations/25543119 | http://purl.uniprot.org/core/pages | "91-97"xsd:string |
| http://purl.uniprot.org/citations/25543119 | http://purl.uniprot.org/core/title | "Identification of MANF as a protein interacting with RTN1-C."xsd:string |
| http://purl.uniprot.org/citations/25543119 | http://purl.uniprot.org/core/volume | "47"xsd:string |
| http://purl.uniprot.org/citations/25543119 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25543119 |
| http://purl.uniprot.org/citations/25543119 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25543119 |
| http://purl.uniprot.org/uniprot/#_A8K878-mappedCitation-25543119 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25543119 |
| http://purl.uniprot.org/uniprot/#_P55145-mappedCitation-25543119 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25543119 |
| http://purl.uniprot.org/uniprot/P55145 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25543119 |
| http://purl.uniprot.org/uniprot/A8K878 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25543119 |