| http://purl.uniprot.org/citations/25555158 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25555158 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25555158 | http://www.w3.org/2000/01/rdf-schema#comment | "U1 snRNP binds to the 5' exon-intron junction of pre-mRNA and thus plays a crucial role at an early stage of pre-mRNA splicing. We present two crystal structures of engineered U1 sub-structures, which together reveal at atomic resolution an almost complete network of protein-protein and RNA-protein interactions within U1 snRNP, and show how the 5' splice site of pre-mRNA is recognised by U1 snRNP. The zinc-finger of U1-C interacts with the duplex between pre-mRNA and the 5'-end of U1 snRNA. The binding of the RNA duplex is stabilized by hydrogen bonds and electrostatic interactions between U1-C and the RNA backbone around the splice junction but U1-C makes no base-specific contacts with pre-mRNA. The structure, together with RNA binding assays, shows that the selection of 5'-splice site nucleotides by U1 snRNP is achieved predominantly through basepairing with U1 snRNA whilst U1-C fine-tunes relative affinities of mismatched 5'-splice sites."xsd:string |
| http://purl.uniprot.org/citations/25555158 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.04986"xsd:string |
| http://purl.uniprot.org/citations/25555158 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.04986"xsd:string |
| http://purl.uniprot.org/citations/25555158 | http://purl.uniprot.org/core/author | "Kondo Y."xsd:string |
| http://purl.uniprot.org/citations/25555158 | http://purl.uniprot.org/core/author | "Kondo Y."xsd:string |
| http://purl.uniprot.org/citations/25555158 | http://purl.uniprot.org/core/author | "Nagai K."xsd:string |
| http://purl.uniprot.org/citations/25555158 | http://purl.uniprot.org/core/author | "Nagai K."xsd:string |
| http://purl.uniprot.org/citations/25555158 | http://purl.uniprot.org/core/author | "Oubridge C."xsd:string |
| http://purl.uniprot.org/citations/25555158 | http://purl.uniprot.org/core/author | "Oubridge C."xsd:string |
| http://purl.uniprot.org/citations/25555158 | http://purl.uniprot.org/core/author | "van Roon A.M."xsd:string |
| http://purl.uniprot.org/citations/25555158 | http://purl.uniprot.org/core/author | "van Roon A.M."xsd:string |
| http://purl.uniprot.org/citations/25555158 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25555158 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25555158 | http://purl.uniprot.org/core/name | "Elife"xsd:string |
| http://purl.uniprot.org/citations/25555158 | http://purl.uniprot.org/core/name | "Elife"xsd:string |
| http://purl.uniprot.org/citations/25555158 | http://purl.uniprot.org/core/title | "Crystal structure of human U1 snRNP, a small nuclear ribonucleoprotein particle, reveals the mechanism of 5' splice site recognition."xsd:string |
| http://purl.uniprot.org/citations/25555158 | http://purl.uniprot.org/core/title | "Crystal structure of human U1 snRNP, a small nuclear ribonucleoprotein particle, reveals the mechanism of 5' splice site recognition."xsd:string |
| http://purl.uniprot.org/citations/25555158 | http://purl.uniprot.org/core/volume | "4"xsd:string |
| http://purl.uniprot.org/citations/25555158 | http://purl.uniprot.org/core/volume | "4"xsd:string |
| http://purl.uniprot.org/citations/25555158 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25555158 |
| http://purl.uniprot.org/citations/25555158 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25555158 |
| http://purl.uniprot.org/citations/25555158 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25555158 |
| http://purl.uniprot.org/citations/25555158 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25555158 |