| http://purl.uniprot.org/citations/25576923 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25576923 | http://www.w3.org/2000/01/rdf-schema#comment | "We used whole genome microarray analysis to identify potential candidate genes with differential expression in BRAFV600E vs NRASQ61R melanoma cells. We selected, for comparison, a peculiar model based on melanoma clones, isolated from a single tumor characterized by mutually exclusive expression of BRAFV600E and NRASQ61R in different cells. This effort led us to identify two genes, SEMA6A and MICAL1, highly expressed in BRAF-mutant vs NRAS-mutant clones. Real-time PCR, Western blot and immunohistochemistry confirmed preferential expression of Sema6A and Mical1 in BRAFV600E melanoma. Sema6A is a member of the semaphorin family, and it complexes with the plexins to regulate actin cytoskeleton, motility and cell proliferation. Silencing of Sema6A in BRAF-mutant cells caused cytoskeletal remodeling, and loss of stress fibers, that in turn induced cell death. Furthermore, Sema6A depletion caused loss of anchorage-independent growth, inhibition of chemotaxis and invasion. Forced Sema6A overexpression, in NRASQ61R clones, induced anchorage-independent growth, and a significant increase of invasiveness. Mical1, that links Sema/PlexinA signaling, is also a negative regulator of apoptosis. Indeed, Mical-1 depletion in BRAF mutant cells restored MST-1-dependent NDR phosphorylation and promoted a rapid and massive NDR-dependent apoptosis. Overall, our data suggest that Sema6A and Mical1 may represent new potential therapeutic targets in BRAFV600E melanoma."xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.org/dc/terms/identifier | "doi:10.18632/oncotarget.2995"xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/author | "Visca P."xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/author | "Loria R."xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/author | "Gallo E."xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/author | "Leonetti C."xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/author | "Anichini A."xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/author | "Perotti V."xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/author | "Falcioni R."xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/author | "Brizzi M.F."xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/author | "Baldassari P."xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/author | "Mortarini R."xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/author | "Bersani I."xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/author | "Bon G."xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/author | "Porru M."xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/author | "Di Carlo S."xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/name | "Oncotarget"xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/pages | "2779-2793"xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/title | "Sema6A and Mical1 control cell growth and survival of BRAFV600E human melanoma cells."xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://purl.uniprot.org/core/volume | "6"xsd:string |
| http://purl.uniprot.org/citations/25576923 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25576923 |
| http://purl.uniprot.org/citations/25576923 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25576923 |
| http://purl.uniprot.org/uniprot/#_A0A0A0MQU6-mappedCitation-25576923 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25576923 |