| http://purl.uniprot.org/citations/25579584 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25579584 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25579584 | http://www.w3.org/2000/01/rdf-schema#comment | "G4 quadruplexes are stable secondary structures prevalent in DNA and RNA that exhibit diverse regulatory functions. Herein, we describe an in vitro technique using the purified RNA helicase RHAU to unwind a G4 quadruplex identified near the 5' end of the human telomerase RNA (hTR). A synthetic RNA corresponding to the quadruplex forming region of hTR (hTR10-43), as well as a predicted complementary strand (25P1), are combined in a reaction containing the purified helicase and ATP. Reaction products and appropriate controls are resolved by native gel electrophoresis. Gels can be stained using a combination of total RNA and quadruplex-specific dyes to observe the expected quadruplex to duplex conversion. This straightforward method can be extended to study structural changes in other inter- or intramolecular quadruplex containing DNA/RNA molecules with the RHAU helicase or other RNA/DNA remodeling enzymes."xsd:string |
| http://purl.uniprot.org/citations/25579584 | http://purl.org/dc/terms/identifier | "doi:10.1007/978-1-4939-2214-7_9"xsd:string |
| http://purl.uniprot.org/citations/25579584 | http://purl.org/dc/terms/identifier | "doi:10.1007/978-1-4939-2214-7_9"xsd:string |
| http://purl.uniprot.org/citations/25579584 | http://purl.uniprot.org/core/author | "Booy E.P."xsd:string |
| http://purl.uniprot.org/citations/25579584 | http://purl.uniprot.org/core/author | "Booy E.P."xsd:string |
| http://purl.uniprot.org/citations/25579584 | http://purl.uniprot.org/core/author | "McKenna S.A."xsd:string |
| http://purl.uniprot.org/citations/25579584 | http://purl.uniprot.org/core/author | "McKenna S.A."xsd:string |
| http://purl.uniprot.org/citations/25579584 | http://purl.uniprot.org/core/author | "McRae E.K."xsd:string |
| http://purl.uniprot.org/citations/25579584 | http://purl.uniprot.org/core/author | "McRae E.K."xsd:string |
| http://purl.uniprot.org/citations/25579584 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25579584 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25579584 | http://purl.uniprot.org/core/name | "Methods Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/25579584 | http://purl.uniprot.org/core/name | "Methods Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/25579584 | http://purl.uniprot.org/core/pages | "125-135"xsd:string |
| http://purl.uniprot.org/citations/25579584 | http://purl.uniprot.org/core/pages | "125-135"xsd:string |
| http://purl.uniprot.org/citations/25579584 | http://purl.uniprot.org/core/title | "Biochemical characterization of G4 quadruplex telomerase RNA unwinding by the RNA helicase RHAU."xsd:string |
| http://purl.uniprot.org/citations/25579584 | http://purl.uniprot.org/core/title | "Biochemical characterization of G4 quadruplex telomerase RNA unwinding by the RNA helicase RHAU."xsd:string |
| http://purl.uniprot.org/citations/25579584 | http://purl.uniprot.org/core/volume | "1259"xsd:string |
| http://purl.uniprot.org/citations/25579584 | http://purl.uniprot.org/core/volume | "1259"xsd:string |
| http://purl.uniprot.org/citations/25579584 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25579584 |
| http://purl.uniprot.org/citations/25579584 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25579584 |
| http://purl.uniprot.org/citations/25579584 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25579584 |
| http://purl.uniprot.org/citations/25579584 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25579584 |