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http://purl.uniprot.org/citations/25580742http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
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Background

Overexpression of connective tissue growth factor (CTGF) in alveolar type II epithelial (AT II) cells disrupts alveolar structure, causes interstitial fibrosis, and upregulates integrin-linked kinase (ILK). Whether CTGF-ILK signaling induces epithelial to mesenchymal transition (EMT) in AT II cells is unknown.

Methods

Transgenic mice with targeted overexpression of CTGF in AT II cells were generated utilizing the surfactant protein C (SP-C) gene promoter and doxycycline-inducible system. AT II cells were isolated from 4-wk-old CTGF-overexpressing (CTGF+) mice and control littermates, and cultured on Matrigel. Cells were transfected with ILK siRNA, and cell morphology and expression of cell differentiation markers were analyzed.

Results

The AT II cells from the control lungs grew in clusters and formed alveolar-like cysts and expressed SP-C. In contrast, the cells from CTGF+ lungs were spread and failed to form alveolar-like cysts. These cells expressed higher levels of CTGF, α smooth muscle actin (α-SMA), fibronectin and vimentin, the mesenchymal markers, suggesting EMT-like changes. Transfection with ILK siRNA not only dramatically attenuated ILK expression, but also decreased α-SMA expression as well as reversed cell morphological changes in CTGF+ AT II cells.

Conclusion

Overexpression of CTGF induces EMT in mouse primary AT II cells and this is mediated by ILK."xsd:string
http://purl.uniprot.org/citations/25580742http://purl.org/dc/terms/identifier"doi:10.1038/pr.2015.8"xsd:string
http://purl.uniprot.org/citations/25580742http://purl.uniprot.org/core/author"Chen S."xsd:string
http://purl.uniprot.org/citations/25580742http://purl.uniprot.org/core/author"Wu S."xsd:string
http://purl.uniprot.org/citations/25580742http://purl.uniprot.org/core/author"Shafieian M."xsd:string
http://purl.uniprot.org/citations/25580742http://purl.uniprot.org/core/date"2015"xsd:gYear
http://purl.uniprot.org/citations/25580742http://purl.uniprot.org/core/name"Pediatr Res"xsd:string
http://purl.uniprot.org/citations/25580742http://purl.uniprot.org/core/pages"520-527"xsd:string
http://purl.uniprot.org/citations/25580742http://purl.uniprot.org/core/title"Integrin-linked kinase mediates CTGF-induced epithelial to mesenchymal transition in alveolar type II epithelial cells."xsd:string
http://purl.uniprot.org/citations/25580742http://purl.uniprot.org/core/volume"77"xsd:string
http://purl.uniprot.org/citations/25580742http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/25580742
http://purl.uniprot.org/citations/25580742http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/25580742
http://purl.uniprot.org/uniprot/#_O55222-mappedCitation-25580742http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/25580742
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