| http://purl.uniprot.org/citations/25601282 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25601282 | http://www.w3.org/2000/01/rdf-schema#comment | "Gastrin is a gastrointestinal hormone secreted by G cells. Hypergastrinemia can improve blood glucose and glycosylated hemoglobin levels. These positive effects are primarily due to the trophic effects of gastrin on β-cells. In recent years, many receptors that regulate secretion of glucagon-like peptide 1 (GLP-1) have been identified in enteroendocrine L cell lines. This led us to hypothesize that, in addition to the trophic effects of gastrin on β-cells, L cells also express cholecystokinin2-receptor (CCK2R), which may regulate GLP-1 secretion and have synergistic effects on glucose homeostasis. Our research provides a preliminary analysis of CCK2R expression and the stimulating effect of gastrin treatment on GLP-1 secretion in a human endocrine L cell line, using RT-PCR, Western blot, immunocytochemistry, and ELISA analyses. The expression of proglucagon and prohormone convertase 3, which regulate GLP-1 biosynthesis, were also analyzed by real-time PCR. Double immunofluorescence labeling was utilized to assess the intracellular localization of CCK2R and GLP-1 in L cells harvested from rat colon tissue. Our results showed that CCK2R was expressed in both the human L cell line and the rat L cells. We also showed that treatment with gastrin, a CCK2R agonist, stimulated the secretion of GLP-1, and that this effect was likely due to increased expression of proglucagon and PCSK1 (also known as prohormone convertase 3 (PC3 gene)). These results not only provide a basis for the role gastrin may play in intestinal L cells, and may also provide the basis for the development of a method of gastrin-mediated glycemic regulation."xsd:string |
| http://purl.uniprot.org/citations/25601282 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.acthis.2014.12.007"xsd:string |
| http://purl.uniprot.org/citations/25601282 | http://purl.uniprot.org/core/author | "Cao Y."xsd:string |
| http://purl.uniprot.org/citations/25601282 | http://purl.uniprot.org/core/author | "Cao X."xsd:string |
| http://purl.uniprot.org/citations/25601282 | http://purl.uniprot.org/core/author | "Liu X.M."xsd:string |
| http://purl.uniprot.org/citations/25601282 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25601282 | http://purl.uniprot.org/core/name | "Acta Histochem"xsd:string |
| http://purl.uniprot.org/citations/25601282 | http://purl.uniprot.org/core/pages | "205-210"xsd:string |
| http://purl.uniprot.org/citations/25601282 | http://purl.uniprot.org/core/title | "Expression of cholecystokinin2-receptor in rat and human L cells and the stimulation of glucagon-like peptide-1 secretion by gastrin treatment."xsd:string |
| http://purl.uniprot.org/citations/25601282 | http://purl.uniprot.org/core/volume | "117"xsd:string |
| http://purl.uniprot.org/citations/25601282 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25601282 |
| http://purl.uniprot.org/citations/25601282 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25601282 |
| http://purl.uniprot.org/uniprot/#_A0A0E3VY36-mappedCitation-25601282 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25601282 |
| http://purl.uniprot.org/uniprot/#_E9PIC8-mappedCitation-25601282 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25601282 |
| http://purl.uniprot.org/uniprot/#_A0SEH4-mappedCitation-25601282 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25601282 |
| http://purl.uniprot.org/uniprot/#_A0SEH5-mappedCitation-25601282 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25601282 |
| http://purl.uniprot.org/uniprot/#_A0SEH6-mappedCitation-25601282 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25601282 |
| http://purl.uniprot.org/uniprot/#_A0SEH7-mappedCitation-25601282 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25601282 |
| http://purl.uniprot.org/uniprot/#_A6HLV7-mappedCitation-25601282 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25601282 |
| http://purl.uniprot.org/uniprot/#_A6I7I2-mappedCitation-25601282 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25601282 |
| http://purl.uniprot.org/uniprot/#_A6I7I3-mappedCitation-25601282 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25601282 |
| http://purl.uniprot.org/uniprot/#_G3V6P5-mappedCitation-25601282 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25601282 |
| http://purl.uniprot.org/uniprot/#_P01350-mappedCitation-25601282 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25601282 |
| http://purl.uniprot.org/uniprot/#_P30553-mappedCitation-25601282 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25601282 |