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http://purl.uniprot.org/citations/25604895http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/25604895http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/25604895http://www.w3.org/2000/01/rdf-schema#comment"

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Modification of wobble uridines of many eukaryotic tRNAs requires the Elongator complex, a highly conserved six-subunit eukaryotic protein assembly, as well as the Killer toxin-insensitive (Kti) proteins 11-14. Kti11 was additionally shown to be implicated in the biosynthesis of diphthamide, a post-translationally modified histidine of translation elongation factor 2. Recent data indicate that iron-bearing Kti11 functions as an electron donor to the [4Fe-4S] cluster of radical S-Adenosylmethionine enzymes, triggering the subsequent radical reaction. We show here that recombinant yeast Kti11 forms a stable 1 : 1 complex with Kti13. To obtain insights into the function of this heterodimer, the Kti11/Kti13 complex was purified to homogeneity, crystallized, and its structure determined at 1.45 Å resolution. The importance of several residues mediating complex formation was confirmed by mutagenesis. Kti13 adopts a fold characteristic of RCC1-like proteins. The seven-bladed β-propeller consists of a unique mixture of four- and three-stranded blades. In the complex, Kti13 orients Kti11 and restricts access to its electron-carrying iron atom, constraining the electron transfer capacity of Kti11. Based on these findings, we propose a role for Kti13, and discuss the possible functional implications of complex formation.

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Structural data have been submitted to the Protein Data Bank under accession number 4X33."xsd:string
http://purl.uniprot.org/citations/25604895http://purl.org/dc/terms/identifier"doi:10.1111/febs.13199"xsd:string
http://purl.uniprot.org/citations/25604895http://purl.org/dc/terms/identifier"doi:10.1111/febs.13199"xsd:string
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/author"Cavarelli J."xsd:string
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/author"Cavarelli J."xsd:string
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/author"McEwen A.G."xsd:string
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/author"McEwen A.G."xsd:string
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/author"Seraphin B."xsd:string
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/author"Seraphin B."xsd:string
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/author"Kolaj-Robin O."xsd:string
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/author"Kolaj-Robin O."xsd:string
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/date"2015"xsd:gYear
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/date"2015"xsd:gYear
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/name"FEBS J."xsd:string
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/name"FEBS J."xsd:string
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/pages"819-833"xsd:string
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/pages"819-833"xsd:string
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/title"Structure of the Elongator cofactor complex Kti11/Kti13 provides insight into the role of Kti13 in Elongator-dependent tRNA modification."xsd:string
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/title"Structure of the Elongator cofactor complex Kti11/Kti13 provides insight into the role of Kti13 in Elongator-dependent tRNA modification."xsd:string
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/volume"282"xsd:string
http://purl.uniprot.org/citations/25604895http://purl.uniprot.org/core/volume"282"xsd:string
http://purl.uniprot.org/citations/25604895http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/25604895
http://purl.uniprot.org/citations/25604895http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/25604895
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