| http://purl.uniprot.org/citations/25633653 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25633653 | http://www.w3.org/2000/01/rdf-schema#comment | "Production of organic acids by microorganisms is of great importance for obtaining building-block chemicals from sustainable biomass. Extracellular accumulation of organic acids involved a series of transporters, which play important roles in the accumulation of specific organic acid while lack of systematic demonstration in eukaryotic microorganisms. To circumvent accumulation of by-product, efforts have being orchestrated to carboxylate transport mechanism for potential clue in Yarrowia lipolytica WSH-Z06. Six endogenous putative transporter genes, YALI0B19470g, YALI0C15488g, YALI0C21406g, YALI0D24607g, YALI0D20108g and YALI0E32901g, were identified. Transport characteristics and substrate specificities were further investigated using a carboxylate-transport-deficient Saccharomyces cerevisiae strain. These transporters were expressed in Y. lipolytica WSH-Z06 to assess their roles in regulating extracellular keto acids accumulation. In a Y. lipolytica T1 line over expressing YALI0B19470g, α-ketoglutarate accumulated to 46.7 g·L(-1), whereas the concentration of pyruvate decreased to 12.3 g·L(-1). Systematic identification of these keto acids transporters would provide clues to further improve the accumulation of specific organic acids with higher efficiency in eukaryotic microorganisms."xsd:string |
| http://purl.uniprot.org/citations/25633653 | http://purl.org/dc/terms/identifier | "doi:10.1038/srep08138"xsd:string |
| http://purl.uniprot.org/citations/25633653 | http://purl.uniprot.org/core/author | "Chen J."xsd:string |
| http://purl.uniprot.org/citations/25633653 | http://purl.uniprot.org/core/author | "Liu P."xsd:string |
| http://purl.uniprot.org/citations/25633653 | http://purl.uniprot.org/core/author | "Guo H."xsd:string |
| http://purl.uniprot.org/citations/25633653 | http://purl.uniprot.org/core/author | "Zhou J."xsd:string |
| http://purl.uniprot.org/citations/25633653 | http://purl.uniprot.org/core/author | "Du G."xsd:string |
| http://purl.uniprot.org/citations/25633653 | http://purl.uniprot.org/core/author | "Madzak C."xsd:string |
| http://purl.uniprot.org/citations/25633653 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25633653 | http://purl.uniprot.org/core/name | "Sci Rep"xsd:string |
| http://purl.uniprot.org/citations/25633653 | http://purl.uniprot.org/core/pages | "8138"xsd:string |
| http://purl.uniprot.org/citations/25633653 | http://purl.uniprot.org/core/title | "Identification and application of keto acids transporters in Yarrowia lipolytica."xsd:string |
| http://purl.uniprot.org/citations/25633653 | http://purl.uniprot.org/core/volume | "5"xsd:string |
| http://purl.uniprot.org/citations/25633653 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25633653 |
| http://purl.uniprot.org/citations/25633653 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25633653 |
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| http://purl.uniprot.org/uniprot/P25613 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25633653 |
| http://purl.uniprot.org/uniprot/P36035 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25633653 |