| http://purl.uniprot.org/citations/25646474 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25646474 | http://www.w3.org/2000/01/rdf-schema#comment | "Activation of an apical Ca(2+)-activated Cl(-) channel (CaCC) triggers the secretion of saliva. It was previously demonstrated that CaCC-mediated Cl(-) current and Cl(-) efflux are absent in the acinar cells of systemic Tmem16A (Tmem16A Cl(-) channel) null mice, but salivation was not assessed in fully developed glands because Tmem16A null mice die within a few days after birth. To test the role of Tmem16A in adult salivary glands, we generated conditional knockout mice lacking Tmem16A in acinar cells (Tmem16A(-/-)). Ca(2+)-dependent salivation was abolished in Tmem16A(-/-) mice, demonstrating that Tmem16A is obligatory for Ca(2+)-mediated fluid secretion. However, the amount of saliva secreted by Tmem16A(-/-) mice in response to the β-adrenergic receptor agonist isoproterenol (IPR) was comparable to that seen in controls, indicating that Tmem16A does not significantly contribute to cAMP-induced secretion. Furthermore, IPR-stimulated secretion was unaffected in mice lacking Cftr (Cftr(∆F508/∆F508)) or ClC-2 (Clcn2(-/-)) Cl(-) channels. The time course for activation of IPR-stimulated fluid secretion closely correlated with that of the IPR-induced cell volume increase, suggesting that acinar swelling may activate a volume-sensitive Cl(-) channel. Indeed, Cl(-) channel blockers abolished fluid secretion, indicating that Cl(-) channel activity is critical for IPR-stimulated secretion. These data suggest that β-adrenergic-induced, cAMP-dependent fluid secretion involves a volume-regulated anion channel. In summary, our results using acinar-specific Tmem16A(-/-) mice identify Tmem16A as the Cl(-) channel essential for muscarinic, Ca(2+)-dependent fluid secretion in adult mouse salivary glands."xsd:string |
| http://purl.uniprot.org/citations/25646474 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1415739112"xsd:string |
| http://purl.uniprot.org/citations/25646474 | http://purl.uniprot.org/core/author | "Choi S."xsd:string |
| http://purl.uniprot.org/citations/25646474 | http://purl.uniprot.org/core/author | "Liu F."xsd:string |
| http://purl.uniprot.org/citations/25646474 | http://purl.uniprot.org/core/author | "Kondo Y."xsd:string |
| http://purl.uniprot.org/citations/25646474 | http://purl.uniprot.org/core/author | "Melvin J.E."xsd:string |
| http://purl.uniprot.org/citations/25646474 | http://purl.uniprot.org/core/author | "Catalan M.A."xsd:string |
| http://purl.uniprot.org/citations/25646474 | http://purl.uniprot.org/core/author | "Shull G.E."xsd:string |
| http://purl.uniprot.org/citations/25646474 | http://purl.uniprot.org/core/author | "Jaramillo Y."xsd:string |
| http://purl.uniprot.org/citations/25646474 | http://purl.uniprot.org/core/author | "Flodby P."xsd:string |
| http://purl.uniprot.org/citations/25646474 | http://purl.uniprot.org/core/author | "Borok Z."xsd:string |
| http://purl.uniprot.org/citations/25646474 | http://purl.uniprot.org/core/author | "Pena-Munzenmayer G."xsd:string |
| http://purl.uniprot.org/citations/25646474 | http://purl.uniprot.org/core/author | "Crandall E."xsd:string |
| http://purl.uniprot.org/citations/25646474 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25646474 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/25646474 | http://purl.uniprot.org/core/pages | "2263-2268"xsd:string |
| http://purl.uniprot.org/citations/25646474 | http://purl.uniprot.org/core/title | "A fluid secretion pathway unmasked by acinar-specific Tmem16A gene ablation in the adult mouse salivary gland."xsd:string |
| http://purl.uniprot.org/citations/25646474 | http://purl.uniprot.org/core/volume | "112"xsd:string |
| http://purl.uniprot.org/citations/25646474 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25646474 |
| http://purl.uniprot.org/citations/25646474 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25646474 |
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| http://purl.uniprot.org/uniprot/#_D3YYA4-mappedCitation-25646474 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25646474 |
| http://purl.uniprot.org/uniprot/#_A0A5F8MPM2-mappedCitation-25646474 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25646474 |
| http://purl.uniprot.org/uniprot/#_A0A2R8VHL2-mappedCitation-25646474 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25646474 |