| http://purl.uniprot.org/citations/25655934 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25655934 | http://www.w3.org/2000/01/rdf-schema#comment | "The G protein-coupled receptor CXCR4 and its ligand stromal-cell derived factor 1 (SDF-1) play a crucial role in directing progenitor cell (PC) homing to ischemic tissue. The Src family protein kinases (SFK) can be activated by, and serve as effectors of, G proteins. In this study we sought to determine whether SFK play a role in SDF-1/CXCR4-mediated PC homing. First, we investigated whether SDF-1/CXCR4 signaling activates SFK. Bone-marrow mononuclear cells (BM MNCs) were isolated from WT and BM-specific CXCR4-KO mice and treated with SDF-1 and/or CXCR4 antagonist AMD3100. SDF-1 treatment rapidly induced phosphorylation (activation) of hematopoietic Src (i.e., Lyn, Fgr, and Hck) in WT cells but not in AMD3100-treated cells or CXCR4-KO cells. Then, we investigated whether SFK are involved in SDF-1/CXCR4-mediated PC chemotaxis. In a combined chemotaxis and endothelial-progenitor-cell (EPC) colony assay, Src inhibitor SU6656 dose-dependently inhibited the SDF-1-induced migration of colony-forming EPCs. Next, we investigated whether SFK play a role in SDF-1/CXCR4-mediated BM PC homing to the ischemic heart. BM MNCs from CXCR4BAC:eGFP reporter mice were i.v. injected into WT and SDF-1BAC:SDF1-RFP transgenic mice following surgically-induced myocardial infarction (MI). eGFP(+) MNCs and eGFP(+)c-kit(+) PCs that were recruited in the infarct border zone in SDF-1BAC:SDF1-RFP recipients were significantly more than that in WT recipients. Treatments of mice with SU6656 significantly reduced eGFP(+) and eGFP(+)c-kit(+) cell recruitment in both WT and SDF-1BAC:RFP recipients and abrogated the difference between the two groups. Remarkably, PCs isolated from BM-specific C-terminal Src kinase (CSK)-KO (Src activated) mice were recruited more efficiently than PCs from WT PCs in the WT recipients. In conclusion, SFK are activated by SDF-1/CXCR4 signaling and play an essential role in SDF-1/CXCR4-mediated BM PC chemotactic response and ischemic cardiac recruitment."xsd:string |
| http://purl.uniprot.org/citations/25655934 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.yjmcc.2015.01.024"xsd:string |
| http://purl.uniprot.org/citations/25655934 | http://purl.uniprot.org/core/author | "Huang K."xsd:string |
| http://purl.uniprot.org/citations/25655934 | http://purl.uniprot.org/core/author | "Cheng M."xsd:string |
| http://purl.uniprot.org/citations/25655934 | http://purl.uniprot.org/core/author | "Zhou J."xsd:string |
| http://purl.uniprot.org/citations/25655934 | http://purl.uniprot.org/core/author | "Tang Y.L."xsd:string |
| http://purl.uniprot.org/citations/25655934 | http://purl.uniprot.org/core/author | "Qin G."xsd:string |
| http://purl.uniprot.org/citations/25655934 | http://purl.uniprot.org/core/author | "Yan D."xsd:string |
| http://purl.uniprot.org/citations/25655934 | http://purl.uniprot.org/core/author | "Miller R.J."xsd:string |
| http://purl.uniprot.org/citations/25655934 | http://purl.uniprot.org/core/author | "Zhao T.C."xsd:string |
| http://purl.uniprot.org/citations/25655934 | http://purl.uniprot.org/core/author | "Losordo D.W."xsd:string |
| http://purl.uniprot.org/citations/25655934 | http://purl.uniprot.org/core/author | "Kishore R."xsd:string |
| http://purl.uniprot.org/citations/25655934 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25655934 | http://purl.uniprot.org/core/name | "J Mol Cell Cardiol"xsd:string |
| http://purl.uniprot.org/citations/25655934 | http://purl.uniprot.org/core/pages | "49-53"xsd:string |
| http://purl.uniprot.org/citations/25655934 | http://purl.uniprot.org/core/title | "A critical role of Src family kinase in SDF-1/CXCR4-mediated bone-marrow progenitor cell recruitment to the ischemic heart."xsd:string |
| http://purl.uniprot.org/citations/25655934 | http://purl.uniprot.org/core/volume | "81"xsd:string |
| http://purl.uniprot.org/citations/25655934 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25655934 |
| http://purl.uniprot.org/citations/25655934 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25655934 |
| http://purl.uniprot.org/uniprot/#_A0A0R4J0N8-mappedCitation-25655934 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25655934 |
| http://purl.uniprot.org/uniprot/#_P70658-mappedCitation-25655934 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25655934 |
| http://purl.uniprot.org/uniprot/#_A1E2I3-mappedCitation-25655934 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25655934 |
| http://purl.uniprot.org/uniprot/#_E9Q2D4-mappedCitation-25655934 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25655934 |
| http://purl.uniprot.org/uniprot/#_P40224-mappedCitation-25655934 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25655934 |