RDF/XMLNTriplesTurtleShow queryShare
SubjectPredicateObject
http://purl.uniprot.org/citations/2567699http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/2567699http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/2567699http://www.w3.org/2000/01/rdf-schema#comment"The polymerase chain reaction was used to clone a full-length human methylmalonyl-CoA mutase cDNA from a human liver library by priming with sequences from the 5' end of a partial cDNA and sequences in the phage vector. The amino acid sequence predicted from the cDNA corresponds to the authentic amino acid sequences of peptide fragment from purified methylmalonyl-CoA mutase. The open reading frame of the cDNA encodes 742 amino acids (82,283 Da) comprising a 32 amino acid mitochondrial leader sequence and a mature protein of 710 amino acids (78,489 Da). The use of the polymerase chain reaction to "screen" the cDNA library represents a novel application of this technique. The full length will enable analysis of mutations underlying inherited methylmalonic acidemias caused by deficiency of the methylmalonyl-CoA mutase apoenzyme."xsd:string
http://purl.uniprot.org/citations/2567699http://purl.org/dc/terms/identifier"doi:10.1016/0888-7543(89)90300-5"xsd:string
http://purl.uniprot.org/citations/2567699http://purl.org/dc/terms/identifier"doi:10.1016/0888-7543(89)90300-5"xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/author"Ledley F.D."xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/author"Ledley F.D."xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/author"Jansen R."xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/author"Jansen R."xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/author"Fenton W.A."xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/author"Fenton W.A."xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/author"Rosenberg L.E."xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/author"Rosenberg L.E."xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/author"Kalousek F."xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/author"Kalousek F."xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/date"1989"xsd:gYear
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/date"1989"xsd:gYear
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/name"Genomics"xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/name"Genomics"xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/pages"198-205"xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/pages"198-205"xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/title"Cloning of full-length methylmalonyl-CoA mutase from a cDNA library using the polymerase chain reaction."xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/title"Cloning of full-length methylmalonyl-CoA mutase from a cDNA library using the polymerase chain reaction."xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/volume"4"xsd:string
http://purl.uniprot.org/citations/2567699http://purl.uniprot.org/core/volume"4"xsd:string