http://purl.uniprot.org/citations/2567699 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/2567699 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/2567699 | http://www.w3.org/2000/01/rdf-schema#comment | "The polymerase chain reaction was used to clone a full-length human methylmalonyl-CoA mutase cDNA from a human liver library by priming with sequences from the 5' end of a partial cDNA and sequences in the phage vector. The amino acid sequence predicted from the cDNA corresponds to the authentic amino acid sequences of peptide fragment from purified methylmalonyl-CoA mutase. The open reading frame of the cDNA encodes 742 amino acids (82,283 Da) comprising a 32 amino acid mitochondrial leader sequence and a mature protein of 710 amino acids (78,489 Da). The use of the polymerase chain reaction to "screen" the cDNA library represents a novel application of this technique. The full length will enable analysis of mutations underlying inherited methylmalonic acidemias caused by deficiency of the methylmalonyl-CoA mutase apoenzyme."xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.org/dc/terms/identifier | "doi:10.1016/0888-7543(89)90300-5"xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.org/dc/terms/identifier | "doi:10.1016/0888-7543(89)90300-5"xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/author | "Ledley F.D."xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/author | "Ledley F.D."xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/author | "Jansen R."xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/author | "Jansen R."xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/author | "Fenton W.A."xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/author | "Fenton W.A."xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/author | "Rosenberg L.E."xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/author | "Rosenberg L.E."xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/author | "Kalousek F."xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/author | "Kalousek F."xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/name | "Genomics"xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/name | "Genomics"xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/pages | "198-205"xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/pages | "198-205"xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/title | "Cloning of full-length methylmalonyl-CoA mutase from a cDNA library using the polymerase chain reaction."xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/title | "Cloning of full-length methylmalonyl-CoA mutase from a cDNA library using the polymerase chain reaction."xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/volume | "4"xsd:string |
http://purl.uniprot.org/citations/2567699 | http://purl.uniprot.org/core/volume | "4"xsd:string |