| http://purl.uniprot.org/citations/25680966 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25680966 | http://www.w3.org/2000/01/rdf-schema#comment | "Methylation of 3'-terminal nucleotides of miRNA/miRNA* is part of miRNAs biogenesis in plants but is not found in animals. In Arabidopsis thaliana this reaction is carried out by a multidomain AdoMet-dependent 2'-O-methyltransferase HEN1. Using deletion and structure-guided mutational analysis, we show that the double-stranded RNA-binding domains R(1) and R(2) of HEN1 make significant but uneven contributions to substrate RNA binding, and map residues in each domain responsible for this function. Using GST pull-down assays and yeast two-hybrid analysis we demonstrate direct HEN1 interactions, mediated by its FK506-binding protein-like domain and R(2) domain, with the microRNA biogenesis protein HYL1. Furthermore, we find that HEN1 forms a complex with DICER-LIKE 1 (DCL1) ribonuclease, another key protein involved in miRNA biogenesis machinery. In contrast, no direct interaction is detectable between HEN1 and SERRATE. On the basis of these findings, we propose a mechanism of plant miRNA maturation which involves binding of the HEN1 methyltransferase to the DCL1•HYL1•miRNA complex excluding the SERRATE protein."xsd:string |
| http://purl.uniprot.org/citations/25680966 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkv102"xsd:string |
| http://purl.uniprot.org/citations/25680966 | http://purl.uniprot.org/core/author | "Venclovas C."xsd:string |
| http://purl.uniprot.org/citations/25680966 | http://purl.uniprot.org/core/author | "Klimasauskas S."xsd:string |
| http://purl.uniprot.org/citations/25680966 | http://purl.uniprot.org/core/author | "Finke A."xsd:string |
| http://purl.uniprot.org/citations/25680966 | http://purl.uniprot.org/core/author | "Vilkaitis G."xsd:string |
| http://purl.uniprot.org/citations/25680966 | http://purl.uniprot.org/core/author | "Plotnikova A."xsd:string |
| http://purl.uniprot.org/citations/25680966 | http://purl.uniprot.org/core/author | "Baranauske S."xsd:string |
| http://purl.uniprot.org/citations/25680966 | http://purl.uniprot.org/core/author | "Mickute M."xsd:string |
| http://purl.uniprot.org/citations/25680966 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25680966 | http://purl.uniprot.org/core/name | "Nucleic Acids Res"xsd:string |
| http://purl.uniprot.org/citations/25680966 | http://purl.uniprot.org/core/pages | "2802-2812"xsd:string |
| http://purl.uniprot.org/citations/25680966 | http://purl.uniprot.org/core/title | "Functional mapping of the plant small RNA methyltransferase: HEN1 physically interacts with HYL1 and DICER-LIKE 1 proteins."xsd:string |
| http://purl.uniprot.org/citations/25680966 | http://purl.uniprot.org/core/volume | "43"xsd:string |
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