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http://purl.uniprot.org/citations/25701318http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/25701318http://www.w3.org/2000/01/rdf-schema#comment"

Background and aim

The roles of host glycosylation in interactions with EPEC and EHEC O157:H7 are largely unclear; this study examined whether O-glycans are involved in EPEC and EHEC O157:H7 adherence to HT-29 cells.

Methods

Bacterial adherence to the cultured cells was determined using the direct co-staining of adherent bacteria and host cells, the adherent bacteria plating, and/or the direct fluorescent observation of the adherent GFP-labeled bacteria.

Results

A comparison of the adherence of EPEC and EHEC O157:H7 to HT-29-Gal and HT-29 cells indicated that the differentiation of HT-29 cells led to a reduction in the adherence of EPEC and EHEC O157:H7. EPEC and EHEC O157:H7 adhesion decreased after the abrogation of O-glycan biosynthesis mediated by benzyl-α-GalNAc treatment. Core 2 O-glycan-deficient HT-29 cells induced by C2GnT2 knockdown had a significant reduction in EPEC and EHEC O157:H7 adhesion in C2GnT2-sh2/HT-29 cells compared with HT-29 and shRNA-Ctr/HT-29 cells. MUC2 expression in benzyl-α-GalNAc-treated HT-29 cells was significantly reduced but unchanged in C2GnT2-deficient HT-29 cells. EPEC or EHEC O157:H7 infection in C2GnT2-deficient HT-29 cells deteriorated the epithelial barrier function. The occludin expression in the shRNA-Ctr/HT-29 and C2GnT2-sh2/HT-29 cells after infection with EPEC or EHEC O157:H7 was pyknic and discontinuous at the cell surface compared with its continuous distribution of control cells. These data indicate that EPEC and EHEC O157:H7 adherence to HT-29 cells is related to mucin-type core 2 O-glycan.

Conclusions

This study provides the concepts toward the design of carbohydrate-dependent inhibition of EPEC and EHEC O157:H7 adhesion to human intestinal epithelial cells."xsd:string
http://purl.uniprot.org/citations/25701318http://purl.org/dc/terms/identifier"doi:10.1007/s10620-015-3548-5"xsd:string
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/author"Chen L."xsd:string
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/author"Chen W."xsd:string
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/author"He Y."xsd:string
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/author"Liu Y."xsd:string
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/author"Li S."xsd:string
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/author"Shang Y."xsd:string
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/author"Tian Y."xsd:string
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/author"Song L."xsd:string
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/author"Ye J."xsd:string
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/author"Wang R."xsd:string
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/author"Zhong X."xsd:string
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/author"Pan Q."xsd:string
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/author"Peng Z."xsd:string
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/date"2015"xsd:gYear
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/name"Dig Dis Sci"xsd:string
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/pages"1977-1990"xsd:string
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/title"Core 2 Mucin-Type O-Glycan Is Related to EPEC and EHEC O157:H7 Adherence to Human Colon Carcinoma HT-29 Epithelial Cells."xsd:string
http://purl.uniprot.org/citations/25701318http://purl.uniprot.org/core/volume"60"xsd:string
http://purl.uniprot.org/citations/25701318http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/25701318
http://purl.uniprot.org/citations/25701318http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/25701318
http://purl.uniprot.org/uniprot/#_B2R758-mappedCitation-25701318http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/25701318
http://purl.uniprot.org/uniprot/#_O95395-mappedCitation-25701318http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/25701318
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