| http://purl.uniprot.org/citations/25728924 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25728924 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25728924 | http://www.w3.org/2000/01/rdf-schema#comment | "Latrophilins, receptors for spider venom α-latrotoxin, are adhesion type G-protein-coupled receptors with emerging functions in synapse development. The N-terminal region binds the endogenous cell adhesion molecule FLRT, a major regulator of cortical and synapse development. We present crystallographic data for the mouse Latrophilin3 lectin and olfactomedin-like (Olf) domains, thereby revealing the Olf β-propeller fold and conserved calcium-binding site. We locate the FLRT-Latrophilin binding surfaces by a combination of sequence conservation analysis, point mutagenesis, and surface plasmon resonance experiments. In stripe assays, we show that wild-type Latrophilin3 and its high-affinity interactor FLRT2, but not the binding-impaired mutants we generated, promote HeLa cell adhesion. In contrast, cortical neurons expressing endogenous FLRTs are repelled by wild-type Latrophilin3 and not by the binding-impaired mutant. Taken together, we present molecular level insights into Latrophilin structure, its FLRT-binding mechanism, and a role for Latrophilin and FLRT that goes beyond a simply adhesive interaction."xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.str.2015.01.013"xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.str.2015.01.013"xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/author | "Klein R."xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/author | "Klein R."xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/author | "Roversi P."xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/author | "Roversi P."xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/author | "Harlos K."xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/author | "Harlos K."xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/author | "Carrasquero M."xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/author | "Carrasquero M."xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/author | "Jackson V.A."xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/author | "Jackson V.A."xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/author | "Seiradake E."xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/author | "Seiradake E."xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/author | "del Toro D."xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/author | "del Toro D."xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/name | "Structure"xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/name | "Structure"xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/pages | "774-781"xsd:string |
| http://purl.uniprot.org/citations/25728924 | http://purl.uniprot.org/core/pages | "774-781"xsd:string |