| http://purl.uniprot.org/citations/25732826 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25732826 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25732826 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/25732826 | http://www.w3.org/2000/01/rdf-schema#comment | "N-terminal acetylation is a major and vital protein modification catalyzed by N-terminal acetyltransferases (NATs). NatF, or Nα-acetyltransferase 60 (Naa60), was recently identified as a NAT in multicellular eukaryotes. Here, we find that Naa60 differs from all other known NATs by its Golgi localization. A new membrane topology assay named PROMPT and a selective membrane permeabilization assay established that Naa60 faces the cytosolic side of intracellular membranes. An Nt-acetylome analysis of NAA60-knockdown cells revealed that Naa60, as opposed to other NATs, specifically acetylates transmembrane proteins and has a preference for N termini facing the cytosol. Moreover, NAA60 knockdown causes Golgi fragmentation, indicating an important role in the maintenance of the Golgi's structural integrity. This work identifies a NAT associated with membranous compartments and establishes N-terminal acetylation as a common modification among transmembrane proteins, a thus-far poorly characterized part of the N-terminal acetylome."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.celrep.2015.01.053"xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.celrep.2015.01.053"xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Arnesen T."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Arnesen T."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Gevaert K."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Gevaert K."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Hole K."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Hole K."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Kalvik T.V."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Kalvik T.V."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Van Damme P."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Van Damme P."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Stoeve S.I."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Stoeve S.I."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Aksnes H."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Aksnes H."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Glomnes N."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Glomnes N."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Marie M."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Marie M."xsd:string |
| http://purl.uniprot.org/citations/25732826 | http://purl.uniprot.org/core/author | "Niere M."xsd:string |