| http://purl.uniprot.org/citations/25732830 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25732830 | http://www.w3.org/2000/01/rdf-schema#comment | "Genetically encoded effectors are important tools for probing cellular function in living animals, but improved methods for directing their expression to specific cell types are required. Here, we introduce a simple, versatile method for achieving cell-type-specific expression of transgenes that leverages the untapped potential of "coding introns" (i.e., introns between coding exons). Our method couples the expression of a transgene to that of a native gene expressed in the cells of interest using intronically inserted "plug-and-play" cassettes (called "Trojan exons") that carry a splice acceptor site followed by the coding sequences of T2A peptide and an effector transgene. We demonstrate the efficacy of this approach in Drosophila using lines containing suitable MiMIC (Minos-mediated integration cassette) transposons and a palette of Trojan exons capable of expressing a range of commonly used transcription factors. We also introduce an exchangeable, MiMIC-like Trojan exon construct that can be targeted to coding introns using the Crispr/Cas system."xsd:string |
| http://purl.uniprot.org/citations/25732830 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.celrep.2015.01.059"xsd:string |
| http://purl.uniprot.org/citations/25732830 | http://purl.uniprot.org/core/author | "Luan H."xsd:string |
| http://purl.uniprot.org/citations/25732830 | http://purl.uniprot.org/core/author | "Potter C.J."xsd:string |
| http://purl.uniprot.org/citations/25732830 | http://purl.uniprot.org/core/author | "Ewer J."xsd:string |
| http://purl.uniprot.org/citations/25732830 | http://purl.uniprot.org/core/author | "Diao F."xsd:string |
| http://purl.uniprot.org/citations/25732830 | http://purl.uniprot.org/core/author | "White B.H."xsd:string |
| http://purl.uniprot.org/citations/25732830 | http://purl.uniprot.org/core/author | "Landgraf M."xsd:string |
| http://purl.uniprot.org/citations/25732830 | http://purl.uniprot.org/core/author | "Marr E."xsd:string |
| http://purl.uniprot.org/citations/25732830 | http://purl.uniprot.org/core/author | "Shropshire W.C."xsd:string |
| http://purl.uniprot.org/citations/25732830 | http://purl.uniprot.org/core/author | "Diao F.'"xsd:string |
| http://purl.uniprot.org/citations/25732830 | http://purl.uniprot.org/core/author | "Ironfield H."xsd:string |
| http://purl.uniprot.org/citations/25732830 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25732830 | http://purl.uniprot.org/core/name | "Cell Rep"xsd:string |
| http://purl.uniprot.org/citations/25732830 | http://purl.uniprot.org/core/pages | "1410-1421"xsd:string |
| http://purl.uniprot.org/citations/25732830 | http://purl.uniprot.org/core/title | "Plug-and-play genetic access to drosophila cell types using exchangeable exon cassettes."xsd:string |
| http://purl.uniprot.org/citations/25732830 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/25732830 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25732830 |
| http://purl.uniprot.org/citations/25732830 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25732830 |
| http://purl.uniprot.org/uniprot/#_A0A0B4KEJ3-mappedCitation-25732830 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25732830 |
| http://purl.uniprot.org/uniprot/#_A8DY91-mappedCitation-25732830 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25732830 |
| http://purl.uniprot.org/uniprot/#_A0A0S0WIG6-mappedCitation-25732830 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25732830 |
| http://purl.uniprot.org/uniprot/#_A1Z858-mappedCitation-25732830 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25732830 |
| http://purl.uniprot.org/uniprot/#_A0A6F7SN88-mappedCitation-25732830 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25732830 |