| http://purl.uniprot.org/citations/25781974 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25781974 | http://www.w3.org/2000/01/rdf-schema#comment | "Syncytin-2 is encoded by the envelope gene of Endogenous Retrovirus-FRD (ERVFRD-1) and plays a critical role in fusion of placental trophoblasts leading to the formation of the multinucleated syncytiotrophoblast. Its expression is consequently regulated in a strict manner. In the present study, we have identified a forskolin-responsive region located between positions -300 to -150 in the Syncytin-2 promoter region. This 150 bp region in the context of a minimal promoter mediated an 80-fold induction of promoter activity following forskolin stimulation. EMSA analyses with competition experiments with nuclear extracts from forskolin-stimulated BeWo cells demonstrated that the -211 to -177 region specifically bound two forskolin-induced complexes, one of them containing a CRE/AP-1-like motif. Site-directed mutagenesis of the CRE/AP-1 binding site in the context of the Syncytin-2 promoter or a heterologous promoter showed that this motif was mostly essential for forskolin-induced promoter activity. Transfection experiments with dominant negative mutants and constitutively activated CREB expression vectors in addition to Chromatin Immunoprecipitation suggested that a CREB family member, CREB2 was binding and acting through the CRE/AP-1 motif. We further demonstrated the binding of JunD to this same motif. Similar to forskolin and soluble cAMP, CREB2 and JunD overexpression induced Syncytin-2 promoter activity in a CRE/AP-1-dependent manner and Syncytin-2 expression. In addition, BeWo cell fusion was induced by both CREB2 and JunD overexpression, while being repressed following silencing of either gene. These results thereby demonstrate that induced expression of Syncytin-2 is highly dependent on the interaction of bZIP-containing transcription factors to a CRE/AP-1 motif and that this element is important for the regulation of Syncytin-2 expression, which results in the formation of the peripheral syncytiotrophoblast layer."xsd:string |
| http://purl.uniprot.org/citations/25781974 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0121468"xsd:string |
| http://purl.uniprot.org/citations/25781974 | http://purl.uniprot.org/core/author | "Vargas A."xsd:string |
| http://purl.uniprot.org/citations/25781974 | http://purl.uniprot.org/core/author | "Rassart E."xsd:string |
| http://purl.uniprot.org/citations/25781974 | http://purl.uniprot.org/core/author | "Barbeau B."xsd:string |
| http://purl.uniprot.org/citations/25781974 | http://purl.uniprot.org/core/author | "Toufaily C."xsd:string |
| http://purl.uniprot.org/citations/25781974 | http://purl.uniprot.org/core/author | "Lokossou A.G."xsd:string |
| http://purl.uniprot.org/citations/25781974 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25781974 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/25781974 | http://purl.uniprot.org/core/pages | "e0121468"xsd:string |
| http://purl.uniprot.org/citations/25781974 | http://purl.uniprot.org/core/title | "A CRE/AP-1-like motif is essential for induced syncytin-2 expression and fusion in human trophoblast-like model."xsd:string |
| http://purl.uniprot.org/citations/25781974 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/25781974 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25781974 |
| http://purl.uniprot.org/citations/25781974 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25781974 |
| http://purl.uniprot.org/uniprot/#_P60508-mappedCitation-25781974 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25781974 |
| http://purl.uniprot.org/uniprot/#_Q8NC12-mappedCitation-25781974 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25781974 |
| http://purl.uniprot.org/uniprot/Q8NC12 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25781974 |
| http://purl.uniprot.org/uniprot/P60508 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25781974 |