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http://purl.uniprot.org/citations/25792650http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/25792650http://www.w3.org/2000/01/rdf-schema#comment"

Aim

The purpose of this study was to investigate the gene expression levels of elastin and fibulin-5 according to differences between carotid plaque regions and to correlate it with clinical features of plaque destabilization.

Materials and methods

The study included 44 endarterectomy specimens available from operated symptomatic carotid artery stenoses. The specimens were separated according to anatomic location: internal carotid artery (ICA), external carotid artery (ECA) and common carotid artery (CCA), and then stored in liquid nitrogen. The amounts of cDNA for elastin and fibulin-5 were determined by Quantitative real-time PCR (Q-RT-PCR). Target gene copy numbers were normalized using hypoxanthine-guanine phosphoribosyltransferase (HPRT1) gene. The delta-delta CT method was applied for relative quantification.

Results

Q-RT-PCR data showed that relative fibulin-5 gene expression was increased in ICA plaque regions when compared to CCA regions but not reaching significance (p=0.061). At the same time, no differences were observed in elastin mRNA level between different anatomic plaque regions (p>0.05). Moreover, elastin and fibulin-5 mRNA expression and clinical parameters were compared in ICA plaques versus CCA and ECA regions, respectively. Up-regulation of elastin and fibulin-5 mRNA levels in ICA were strongly correlated with family history of cardiovascular disease when compared to CCA (p<0.05). Up-regulation of fibulin-5 in ICA was significantly associated with diabetes, and elevated triglycerides and very low density lipoprotein (VLDL) when compared to ECA (p<0.05).

Conclusion

The clinical significance is the differences between the proximal and distal regions of the lesion, associated with the ICA, CCA and ECA respectively, with increased fibulin-5 in the ICA region."xsd:string
http://purl.uniprot.org/citations/25792650http://purl.uniprot.org/core/author"Aksoy M."xsd:string
http://purl.uniprot.org/citations/25792650http://purl.uniprot.org/core/author"Isbir T."xsd:string
http://purl.uniprot.org/citations/25792650http://purl.uniprot.org/core/author"Ergen A."xsd:string
http://purl.uniprot.org/citations/25792650http://purl.uniprot.org/core/author"Zeybek U."xsd:string
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http://purl.uniprot.org/citations/25792650http://purl.uniprot.org/core/author"Sivrikoz E."xsd:string
http://purl.uniprot.org/citations/25792650http://purl.uniprot.org/core/author"Yanar F."xsd:string
http://purl.uniprot.org/citations/25792650http://purl.uniprot.org/core/date"2015"xsd:gYear
http://purl.uniprot.org/citations/25792650http://purl.uniprot.org/core/name"In Vivo"xsd:string
http://purl.uniprot.org/citations/25792650http://purl.uniprot.org/core/pages"229-235"xsd:string
http://purl.uniprot.org/citations/25792650http://purl.uniprot.org/core/title"Gene expression levels of elastin and fibulin-5 according to differences between carotid plaque regions."xsd:string
http://purl.uniprot.org/citations/25792650http://purl.uniprot.org/core/volume"29"xsd:string
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