| http://purl.uniprot.org/citations/25813404 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25813404 | http://www.w3.org/2000/01/rdf-schema#comment | "Detection of ALK rearrangements in patients with non-small cell lung cancer (NSCLC) presents a significant technical challenge due to the existence of multiple translocation partners and break-points. To improve the performance of PCR-based tests, we utilized the combination of 2 assays, i.e. the variant-specific PCR for the 5 most common ALK rearrangements and the test for unbalanced 5'/3'-end ALK expression. Overall, convincing evidence for the presence of ALK translocation was obtained for 34/400 (8.5%) cases, including 14 EML4ex13/ALKex20, 12 EML4ex6/ALKex20, 3 EML4ex18/ALKex20, 2 EML4ex20/ALKex20 variants and 3 tumors with novel translocation partners. 386 (96.5%) out of 400 EGFR mutation-negative NSCLCs were concordant for both tests, being either positive (n = 26) or negative (n = 360) for ALK translocation; 49 of these samples (6 ALK+, 43 ALK-) were further evaluated by FISH, and there were no instances of disagreement. Among the 14 (3.5%) "discordant" tumors, 5 demonstrated ALK translocation by the first but not by the second PCR assay, and 9 had unbalanced ALK expression in the absence of known ALK fusion variants. 5 samples from the latter group were subjected to FISH, and the presence of translocation was confirmed in 2 cases. Next generation sequencing analysis of these 2 samples identified novel translocation partners, DCTN1 and SQSTM1; furthermore, the DCTN1/ALK fusion was also found in another NSCLC sample with unbalanced 5'/3'-end ALK expression, indicating a recurrent nature of this translocation. We conclude that the combination of 2 different PCR tests is a viable approach for the diagnostics of ALK rearrangements. Systematic typing of ALK fusions is likely to reveal new NSCLC-specific ALK partners."xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.canlet.2015.03.028"xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/author | "Imyanitov E.N."xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/author | "Kuligina E.S.h."xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/author | "Togo A.V."xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/author | "Pozharisski K.M."xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/author | "Iyevleva A.G."xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/author | "Sokolenko A.P."xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/author | "Mitiushkina N.V."xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/author | "Ivantsov A.O."xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/author | "Aleksakhina S.N."xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/author | "Tiurin V.I."xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/author | "Strelkova T.N."xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/author | "Garifullina A.R."xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/author | "Merkulov V.O."xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/author | "Raskin G.A."xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/name | "Cancer Lett"xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/pages | "116-121"xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/title | "Novel ALK fusion partners in lung cancer."xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://purl.uniprot.org/core/volume | "362"xsd:string |
| http://purl.uniprot.org/citations/25813404 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25813404 |
| http://purl.uniprot.org/citations/25813404 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25813404 |
| http://purl.uniprot.org/uniprot/#_A0A0K2YUJ3-mappedCitation-25813404 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25813404 |