| http://purl.uniprot.org/citations/25814554 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25814554 | http://www.w3.org/2000/01/rdf-schema#comment | "Post-translational protein modifications, such as tyrosine phosphorylation, regulate protein-protein interactions (PPIs) critical for signal processing and cellular phenotypes. We extended an established yeast two-hybrid system employing human protein kinases for the analyses of phospho-tyrosine (pY)-dependent PPIs in a direct experimental, large-scale approach. We identified 292 mostly novel pY-dependent PPIs which showed high specificity with respect to kinases and interacting proteins and validated a large fraction in co-immunoprecipitation experiments from mammalian cells. About one-sixth of the interactions are mediated by known linear sequence binding motifs while the majority of pY-PPIs are mediated by other linear epitopes or governed by alternative recognition modes. Network analysis revealed that pY-mediated recognition events are tied to a highly connected protein module dedicated to signaling and cell growth pathways related to cancer. Using binding assays, protein complementation and phenotypic readouts to characterize the pY-dependent interactions of TSPAN2 (tetraspanin 2) and GRB2 or PIK3R3 (p55γ), we exemplarily provide evidence that the two pY-dependent PPIs dictate cellular cancer phenotypes."xsd:string |
| http://purl.uniprot.org/citations/25814554 | http://purl.org/dc/terms/identifier | "doi:10.15252/msb.20145968"xsd:string |
| http://purl.uniprot.org/citations/25814554 | http://purl.uniprot.org/core/author | "Stelzl U."xsd:string |
| http://purl.uniprot.org/citations/25814554 | http://purl.uniprot.org/core/author | "Benlasfer N."xsd:string |
| http://purl.uniprot.org/citations/25814554 | http://purl.uniprot.org/core/author | "Birth P."xsd:string |
| http://purl.uniprot.org/citations/25814554 | http://purl.uniprot.org/core/author | "Grossmann A."xsd:string |
| http://purl.uniprot.org/citations/25814554 | http://purl.uniprot.org/core/author | "Apelt L."xsd:string |
| http://purl.uniprot.org/citations/25814554 | http://purl.uniprot.org/core/author | "Hegele A."xsd:string |
| http://purl.uniprot.org/citations/25814554 | http://purl.uniprot.org/core/author | "Wachsmuth F."xsd:string |
| http://purl.uniprot.org/citations/25814554 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25814554 | http://purl.uniprot.org/core/name | "Mol Syst Biol"xsd:string |
| http://purl.uniprot.org/citations/25814554 | http://purl.uniprot.org/core/pages | "794"xsd:string |
| http://purl.uniprot.org/citations/25814554 | http://purl.uniprot.org/core/title | "Phospho-tyrosine dependent protein-protein interaction network."xsd:string |
| http://purl.uniprot.org/citations/25814554 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/25814554 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25814554 |
| http://purl.uniprot.org/citations/25814554 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25814554 |
| http://purl.uniprot.org/uniprot/O43559#attribution-0B42C0F8F11604089F71FC9199786C22 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/25814554 |
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| http://purl.uniprot.org/uniprot/#_P52757-mappedCitation-25814554 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25814554 |
| http://purl.uniprot.org/uniprot/#_P61371-mappedCitation-25814554 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25814554 |
| http://purl.uniprot.org/uniprot/#_Q07869-mappedCitation-25814554 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25814554 |
| http://purl.uniprot.org/uniprot/#_Q07912-mappedCitation-25814554 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25814554 |
| http://purl.uniprot.org/uniprot/#_Q14141-mappedCitation-25814554 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25814554 |
| http://purl.uniprot.org/uniprot/#_Q15027-mappedCitation-25814554 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25814554 |