| http://purl.uniprot.org/citations/25823913 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25823913 | http://www.w3.org/2000/01/rdf-schema#comment | "Reduced expression in immortalized cells (REIC)/Dickkopf (Dkk)-3 is a tumor-suppressor gene and has been studied as a promising therapeutic gene for cancer gene therapy. Intratumoral injection of an adenovirus vector carrying the human REIC/Dkk-3 gene (Ad-REIC) elicits cancer cell-specific apoptosis and anticancer immune responses. The cytokine-like effect of secretory REIC/Dkk-3 on the induction of dendritic cell (DC)-like cell differentiation from monocytes plays a role in systemic anticancer immunity. In the present study, we generated recombinant full-length and N-terminally truncated REIC/Dkk-3 to characterize the biological activity of the protein. During the purification procedure, we identified a 17 kDa cysteine-rich stable product (C17-REIC) showing limited degradation. Further analysis showed that the C17-REIC domain was sufficient for the induction of DC-like cell differentiation from monocytes. Concomitant with the differentiation of DCs, the REIC/Dkk-3 protein induced the phosphorylation of glycogen synthase kinase 3β (GSK-3β) and signal transducers and activators of transcription (STAT) at a level comparable to that of granulocyte/macrophage colony-stimulating factor. In a mouse model of subcutaneous renal adenocarcinoma, intraperitoneal injection of full-length and C17-REIC proteins exerted anticancer effects in parallel with the activation of immunocompetent cells such as DCs and cytotoxic T lymphocytes in peripheral blood. Taken together, our results indicate that the stable cysteine-rich core region of REIC/Dkk-3 is responsible for the induction of anticancer immune responses. Because REIC/Dkk-3 is a naturally circulating serum protein, the upregulation REIC/Dkk-3 protein expression could be a promising option for cancer therapy."xsd:string |
| http://purl.uniprot.org/citations/25823913 | http://purl.org/dc/terms/identifier | "doi:10.3892/or.2015.3885"xsd:string |
| http://purl.uniprot.org/citations/25823913 | http://purl.uniprot.org/core/author | "Huang P."xsd:string |
| http://purl.uniprot.org/citations/25823913 | http://purl.uniprot.org/core/author | "Sakaguchi M."xsd:string |
| http://purl.uniprot.org/citations/25823913 | http://purl.uniprot.org/core/author | "Watanabe M."xsd:string |
| http://purl.uniprot.org/citations/25823913 | http://purl.uniprot.org/core/author | "Kumon H."xsd:string |
| http://purl.uniprot.org/citations/25823913 | http://purl.uniprot.org/core/author | "Li S.A."xsd:string |
| http://purl.uniprot.org/citations/25823913 | http://purl.uniprot.org/core/author | "Futami J."xsd:string |
| http://purl.uniprot.org/citations/25823913 | http://purl.uniprot.org/core/author | "Kinoshita R."xsd:string |
| http://purl.uniprot.org/citations/25823913 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25823913 | http://purl.uniprot.org/core/name | "Oncol Rep"xsd:string |
| http://purl.uniprot.org/citations/25823913 | http://purl.uniprot.org/core/pages | "2908-2914"xsd:string |
| http://purl.uniprot.org/citations/25823913 | http://purl.uniprot.org/core/title | "The cysteine-rich core domain of REIC/Dkk-3 is critical for its effect on monocyte differentiation and tumor regression."xsd:string |
| http://purl.uniprot.org/citations/25823913 | http://purl.uniprot.org/core/volume | "33"xsd:string |
| http://purl.uniprot.org/citations/25823913 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25823913 |
| http://purl.uniprot.org/citations/25823913 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25823913 |
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| http://purl.uniprot.org/uniprot/#_Q9UBP4-mappedCitation-25823913 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25823913 |