http://purl.uniprot.org/citations/25824796 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/25824796 | http://www.w3.org/2000/01/rdf-schema#comment | "PurposeTo investigate effects of the TESTIN (TES) gene on proliferation and migration of highly metastatic nasopharyngeal carcinoma cell line 5-8F and the related mechanisms.Materials and methodsThe target gene of human nasopharyngeal carcinoma cell line 5-8F was amplified by PCR and cloned into the empty plasmid pEGFP-N1 to construct a eukaryotic expression vector pEGFP-N1-TES. This was then transfected into 5-8F cells. MTT assays, flow cytometry and scratch wound tests were used to detect the proliferation and migration of transfected 5-8F cells.ResultsA cell model with stable and high expression of TES gene was successfully established. MTT assays showed that the OD value of 5-8F/TES cells was markedly lower than that of 5-8F/GFP cells and 5-8F cells (p<0.05). Flow cytometry showed that the apoptosis rate of 5-8F/TES cells was prominently increased compared with 5-8F/GFP cells and 5-8F cells (p<0.05). In vitro scratch wound assays showed that, the width of the wound area of 5-8F/TES cells narrowed slightly, while the width of the wound area of 5-8F/ GFP cells and 5-8F cells narrowed sharply, suggesting that the TES overexpression could inhibit the migration ability.ConclusionsTES gene expression remarkably inhibits the proliferation of human nasopharyngeal carcinoma cell line 5-8F and reduces its migration in vitro. Thus, it may be a potential tumor suppressor gene for nasopharyngeal carcinoma."xsd:string |
http://purl.uniprot.org/citations/25824796 | http://purl.org/dc/terms/identifier | "doi:10.7314/apjcp.2015.16.6.2555"xsd:string |
http://purl.uniprot.org/citations/25824796 | http://purl.uniprot.org/core/author | "Zhang F."xsd:string |
http://purl.uniprot.org/citations/25824796 | http://purl.uniprot.org/core/author | "Zhong Z."xsd:string |
http://purl.uniprot.org/citations/25824796 | http://purl.uniprot.org/core/author | "Yin S.C."xsd:string |
http://purl.uniprot.org/citations/25824796 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
http://purl.uniprot.org/citations/25824796 | http://purl.uniprot.org/core/name | "Asian Pac J Cancer Prev"xsd:string |
http://purl.uniprot.org/citations/25824796 | http://purl.uniprot.org/core/pages | "2555-2559"xsd:string |
http://purl.uniprot.org/citations/25824796 | http://purl.uniprot.org/core/title | "Effects of TESTIN gene expression on proliferation and migration of the 5-8F nasopharyngeal carcinoma cell line."xsd:string |
http://purl.uniprot.org/citations/25824796 | http://purl.uniprot.org/core/volume | "16"xsd:string |
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