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http://purl.uniprot.org/citations/25825759http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/25825759http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/25825759http://www.w3.org/2000/01/rdf-schema#comment"The discovery of African henipaviruses (HNVs) related to pathogenic Hendra virus (HeV) and Nipah virus (NiV) from Southeast Asia and Australia presents an open-ended health risk. Cell receptor use by emerging African HNVs at the stage of host-cell entry is a key parameter when considering the potential for spillover and infection of human populations. The attachment glycoprotein from a Ghanaian bat isolate (GhV-G) exhibits <30% sequence identity with Asiatic NiV-G/HeV-G. Here, through functional and structural analysis of GhV-G, we show how this African HNV targets the same human cell-surface receptor (ephrinB2) as the Asiatic HNVs. We first characterized this virus-receptor interaction crystallographically. Compared with extant HNV-G-ephrinB2 structures, there was significant structural variation in the six-bladed β-propeller scaffold of the GhV-G receptor-binding domain, but not the Greek key fold of the bound ephrinB2. Analysis revealed a surprisingly conserved mode of ephrinB2 interaction that reflects an ongoing evolutionary constraint among geographically distal and phylogenetically divergent HNVs to maintain the functionality of ephrinB2 recognition during virus-host entry. Interestingly, unlike NiV-G/HeV-G, we could not detect binding of GhV-G to ephrinB3. Comparative structure-function analysis further revealed several distinguishing features of HNV-G function: a secondary ephrinB2 interaction site that contributes to more efficient ephrinB2-mediated entry in NiV-G relative to GhV-G and cognate residues at the very C terminus of GhV-G (absent in Asiatic HNV-Gs) that are vital for efficient receptor-induced fusion, but not receptor binding per se. These data provide molecular-level details for evaluating the likelihood of African HNVs to spill over into human populations."xsd:string
http://purl.uniprot.org/citations/25825759http://purl.org/dc/terms/identifier"doi:10.1073/PNAS.1501690112"xsd:string
http://purl.uniprot.org/citations/25825759http://purl.org/dc/terms/identifier"doi:10.1073/pnas.1501690112"xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/author"Lee B."xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/author"Lee B."xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/author"Ahmed A.A."xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/author"Ahmed A.A."xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/author"Bowden T.A."xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/author"Bowden T.A."xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/author"Zeltina A."xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/author"Zeltina A."xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/author"Beaty S.M."xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/author"Beaty S.M."xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/author"Pernet O."xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/author"Pernet O."xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/date"2015"xsd:gYear
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/date"2015"xsd:gYear
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/name"Proc. Natl. Acad. Sci. U.S.A."xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/name"Proc Natl Acad Sci U S A"xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/pages"E2156-E2165"xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/pages"E2156-65"xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/title"Molecular recognition of human ephrinB2 cell surface receptor by an emergent African henipavirus."xsd:string
http://purl.uniprot.org/citations/25825759http://purl.uniprot.org/core/title"Molecular recognition of human ephrinB2 cell surface receptor by an emergent African henipavirus."xsd:string
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