| http://purl.uniprot.org/citations/25831459 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25831459 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25831459 | http://www.w3.org/2000/01/rdf-schema#comment | "RNF is a redox-driven ion (Na(+) and in one case possibly H(+)) transporter present in many prokaryotes. It has been proposed that RNF performs a variety of reactions in different organisms, delivering low-potential reducing equivalents for specific cellular processes. RNF shares strong homology with the Na(+)-pumping respiratory enzyme Na(+)-NQR, although there are significant differences in subunit and redox cofactor composition. Here we report a topological analysis of the six subunits of RNF from Vibrio cholerae. Although individual subunits from other organisms have previously been studied, this is the first complete, experimentally derived, analysis of RNF from any one source. This has allowed us to identify and confirm key properties of RNF. The putative NADH binding site in RnfC is located on the cytoplasmic side of the membrane. FeS centers in RnfB and RnfC are also located on the cytoplasmic side. However, covalently attached FMNs in RnfD and RnfG are both located in the periplasm. RNF also contains a number of acidic residues that correspond to functionally important groups in Na(+)-NQR. The acidic residues involved in Na(+) uptake and many of those implicated in Na(+) translocation are topologically conserved. The topology of RNF closely matches the topology represented in the newly published structure of Na(+)-NQR, consistent with the close relation between the two enzymes. The topology of RNF is discussed in the context of the current structural model of Na(+)-NQR, and the proposed functionality of the RNF complex itself."xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.org/dc/terms/identifier | "doi:10.1021/acs.biochem.5b00020"xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.org/dc/terms/identifier | "doi:10.1021/acs.biochem.5b00020"xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/author | "Bourges A."xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/author | "Bourges A."xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/author | "Barquera B."xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/author | "Barquera B."xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/author | "Juarez O."xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/author | "Juarez O."xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/author | "Duffy E.B."xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/author | "Duffy E.B."xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/author | "Herce H.D."xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/author | "Herce H.D."xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/author | "Hreha T.N."xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/author | "Hreha T.N."xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/author | "Mezic K.G."xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/author | "Mezic K.G."xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/author | "Pryshchep S."xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/author | "Pryshchep S."xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/25831459 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |