| http://purl.uniprot.org/citations/25838544 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25838544 | http://www.w3.org/2000/01/rdf-schema#comment | "Unlike typical cis-splicing, trans-splicing joins exons from two separate transcripts to produce chimeric mRNA and has been detected in most eukaryotes. Trans-splicing in trypanosomes and nematodes has been characterized as a spliced leader RNA-facilitated reaction; in contrast, its mechanism in higher eukaryotes remains unclear. Here we investigate mod(mdg4), a classic trans-spliced gene in Drosophila, and report that two critical RNA sequences in the middle of the last 5' intron, TSA and TSB, promote trans-splicing of mod(mdg4). In TSA, a 13-nucleotide (nt) core motif is conserved across Drosophila species and is essential and sufficient for trans-splicing, which binds U1 small nuclear RNP (snRNP) through strong base-pairing with U1 snRNA. In TSB, a conserved secondary structure acts as an enhancer. Deletions of TSA and TSB using the CRISPR/Cas9 system result in developmental defects in flies. Although it is not clear how the 5' intron finds the 3' introns, compensatory changes in U1 snRNA rescue trans-splicing of TSA mutants, demonstrating that U1 recruitment is critical to promote trans-splicing in vivo. Furthermore, TSA core-like motifs are found in many other trans-spliced Drosophila genes, including lola. These findings represent a novel mechanism of trans-splicing, in which RNA motifs in the 5' intron are sufficient to bring separate transcripts into close proximity to promote trans-splicing."xsd:string |
| http://purl.uniprot.org/citations/25838544 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.258863.115"xsd:string |
| http://purl.uniprot.org/citations/25838544 | http://purl.uniprot.org/core/author | "Hao J."xsd:string |
| http://purl.uniprot.org/citations/25838544 | http://purl.uniprot.org/core/author | "Li L."xsd:string |
| http://purl.uniprot.org/citations/25838544 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/25838544 | http://purl.uniprot.org/core/author | "Wang X.Y."xsd:string |
| http://purl.uniprot.org/citations/25838544 | http://purl.uniprot.org/core/author | "Pu J."xsd:string |
| http://purl.uniprot.org/citations/25838544 | http://purl.uniprot.org/core/author | "Zhan S."xsd:string |
| http://purl.uniprot.org/citations/25838544 | http://purl.uniprot.org/core/author | "Gao J.L."xsd:string |
| http://purl.uniprot.org/citations/25838544 | http://purl.uniprot.org/core/author | "Xu Y.Z."xsd:string |
| http://purl.uniprot.org/citations/25838544 | http://purl.uniprot.org/core/author | "Shao W."xsd:string |
| http://purl.uniprot.org/citations/25838544 | http://purl.uniprot.org/core/author | "Fan Y.J."xsd:string |
| http://purl.uniprot.org/citations/25838544 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25838544 | http://purl.uniprot.org/core/name | "Genes Dev"xsd:string |
| http://purl.uniprot.org/citations/25838544 | http://purl.uniprot.org/core/pages | "760-771"xsd:string |
| http://purl.uniprot.org/citations/25838544 | http://purl.uniprot.org/core/title | "A conserved intronic U1 snRNP-binding sequence promotes trans-splicing in Drosophila."xsd:string |
| http://purl.uniprot.org/citations/25838544 | http://purl.uniprot.org/core/volume | "29"xsd:string |
| http://purl.uniprot.org/citations/25838544 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25838544 |
| http://purl.uniprot.org/citations/25838544 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25838544 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K6B8-mappedCitation-25838544 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25838544 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K7A6-mappedCitation-25838544 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25838544 |
| http://purl.uniprot.org/uniprot/#_Q867Z4-mappedCitation-25838544 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25838544 |
| http://purl.uniprot.org/uniprot/#_Q86B87-mappedCitation-25838544 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25838544 |
| http://purl.uniprot.org/uniprot/#_Q70BZ0-mappedCitation-25838544 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25838544 |