| http://purl.uniprot.org/citations/25847245 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25847245 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25847245 | http://www.w3.org/2000/01/rdf-schema#comment | "Two DNA damage-inducible genes in Saccharomyces cerevisiae, DDI2 and DDI3, are identical and encode putative HD domain-containing proteins, whose functions are currently unknown. Because Ddi2/3 also shows limited homology to a fungal cyanamide hydratase that converts cyanamide to urea, we tested the enzymatic activity of recombinant Ddi2. To this end, we developed a novel enzymatic assay and determined that the Km value of the recombinant Ddi2/3 for cyanamide is 17.3 ± 0.05 mm, and its activity requires conserved residues in the HD domain. Unlike most other DNA damage-inducible genes, DDI2/3 is only induced by a specific set of alkylating agents and surprisingly is strongly induced by cyanamide. To characterize the biological function of DDI2/3, we sequentially deleted both DDI genes and found that the double mutant was unable to metabolize cyanamide and became much more sensitive to growth inhibition by cyanamide, suggesting that the DDI2/3 genes protect host cells from cyanamide toxicity. Despite the physiological relevance of the cyanamide induction, DDI2/3 is not involved in its own transcriptional regulation. The significance of cyanamide hydratase activity and its induced expression is discussed."xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m115.645408"xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m115.645408"xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/author | "Fu Y."xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/author | "Fu Y."xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/author | "Li J."xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/author | "Li J."xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/author | "Xu X."xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/author | "Xu X."xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/author | "Moore S.A."xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/author | "Moore S.A."xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/author | "Xiao W."xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/author | "Xiao W."xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/author | "Biss M."xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/author | "Biss M."xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/pages | "12664-12675"xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/pages | "12664-12675"xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/title | "Two duplicated genes DDI2 and DDI3 in budding yeast encode a cyanamide hydratase and are induced by cyanamide."xsd:string |
| http://purl.uniprot.org/citations/25847245 | http://purl.uniprot.org/core/title | "Two duplicated genes DDI2 and DDI3 in budding yeast encode a cyanamide hydratase and are induced by cyanamide."xsd:string |