http://purl.uniprot.org/citations/25848690 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/25848690 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/25848690 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundSec18p/N-ethylmaleimide-sensitive factor (NSF) is a conserved eukaryotic ATPase, which primarily functions in vesicle membrane fusion from yeast to human. However, the function of the OsSec18 gene, a homologue of NSF in rice, remains unknown.ResultsIn the present study, we investigated the function of OsSec18 in rice and found that OsSec18 complements the temperature-sensitive phenotype and interferes with vacuolar morphogenesis in yeast. Overexpression of OsSec18 in rice decreased the plant height and 1000-grain weight and altered the morphology of the protein bodies. Further examination revealed that OsSec18 presented as a 290-kDa complex in rice endosperm cells. Moreover, Os60sP0 was identified a component of this complex, demonstrating that the OsSec18 complex contains another complex of P0(P1-P2)2 in rice endosperm cells. Furthermore, we determined that the N-terminus of OsSec18 can interact with the N- and C-termini of Os60sP0, whereas the C-terminus of OsSec18 can only interact with the C-terminus of Os60sP0.ConclusionOur results revealed that the OsSec18 regulates vacuolar morphology in both yeast and rice endosperm cell and the OsSec18 interacts with P0(P1-P2)2 complex in rice endosperm cell."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.org/dc/terms/identifier | "doi:10.1186/s12870-014-0324-1"xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.org/dc/terms/identifier | "doi:10.1186/s12870-014-0324-1"xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/author | "Guo Z."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/author | "Guo Z."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/author | "Liu Z."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/author | "Liu Z."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/author | "Sun Y."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/author | "Sun Y."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/author | "Song G."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/author | "Song G."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/author | "Yang D."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/author | "Yang D."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/author | "Jiang D."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/author | "Jiang D."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/author | "Pang J."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/author | "Pang J."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/author | "Ning T."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/author | "Ning T."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/name | "BMC Plant Biol."xsd:string |
http://purl.uniprot.org/citations/25848690 | http://purl.uniprot.org/core/name | "BMC Plant Biol."xsd:string |