| http://purl.uniprot.org/citations/25851606 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25851606 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25851606 | http://www.w3.org/2000/01/rdf-schema#comment | "In Caenorhabditis elegans, twitchin is a giant polypeptide located in muscle A-bands. The protein kinase of twitchin is autoinhibited by 45 residues upstream (NL) and 60 residues downstream (CRD) of the kinase catalytic core. Molecular dynamics simulation on a twitchin fragment revealed that the NL is released by pulling force. However, it is unclear how the CRD is removed. To identify proteins that may remove the CRD, we performed a yeast two-hybrid screen using twitchin kinase as bait. One interactor is MAK-1, C. elegans orthologue of MAPKAP kinase 2. MAPKAP kinase 2 is phosphorylated and activated by p38 MAP kinase. We demonstrate that the CRD of twitchin is important for binding to MAK-1. mak-1 is expressed in nematode body wall muscle, and antibodies to MAK-1 localize between and around Z-disk analogues and to the edge of A-bands. Whereas unc-22 mutants are completely resistant, mak-1 mutants are partially resistant to nicotine. MAK-1 can phosphorylate twitchin NL-Kin-CRD in vitro. Genetic data suggest the involvement of two other mak-1 paralogues and two orthologues of p38 MAP kinase. These results suggest that MAK-1 is an activator of twitchin kinase and that the p38 MAP kinase pathway may be involved in the regulation of twitchin."xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e14-05-1009"xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e14-05-1009"xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/author | "Qadota H."xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/author | "Qadota H."xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/author | "Furukawa M."xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/author | "Furukawa M."xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/author | "Benian G.M."xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/author | "Benian G.M."xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/author | "Matsunaga Y."xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/author | "Matsunaga Y."xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/author | "Choe H.H."xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/author | "Choe H.H."xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/name | "Mol. Biol. Cell"xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/name | "Mol. Biol. Cell"xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/pages | "2096-2111"xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/pages | "2096-2111"xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/title | "Twitchin kinase interacts with MAPKAP kinase 2 in Caenorhabditis elegans striated muscle."xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/title | "Twitchin kinase interacts with MAPKAP kinase 2 in Caenorhabditis elegans striated muscle."xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/volume | "26"xsd:string |
| http://purl.uniprot.org/citations/25851606 | http://purl.uniprot.org/core/volume | "26"xsd:string |