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| http://purl.uniprot.org/citations/25855754 | http://www.w3.org/2000/01/rdf-schema#comment | "Erythropoiesis, in which committed progenitor cells generate millions of erythrocytes daily, involves dramatic changes in the chromatin structure and transcriptome of erythroblasts, prior to their enucleation. While the involvement of the master-regulatory transcription factors GATA binding protein 1 (GATA-1) and GATA-2 in this process is established, the mechanistic contributions of many chromatin-modifying/remodeling enzymes in red cell biology remain enigmatic. We demonstrated that SetD8, a histone methyltransferase that catalyzes monomethylation of histone H4 at lysine 20 (H4K20me1), is a context-dependent GATA-1 corepressor in erythroid cells. To determine whether SetD8 controls erythroid maturation and/or function, we used a small hairpin RNA (shRNA)-based loss-of-function strategy in a primary murine erythroblast culture system. In this system, SetD8 promoted erythroblast maturation and survival, and this did not involve upregulation of the established regulator of erythroblast survival Bcl-x(L). SetD8 catalyzed H4K20me1 at a critical Gata2 cis element and restricted occupancy by an enhancer of Gata2 transcription, Scl/TAL1, thereby repressing Gata2 transcription. Elevating GATA-2 levels in erythroid precursors yielded a maturation block comparable to that induced by SetD8 downregulation. As lowering GATA-2 expression in the context of SetD8 knockdown did not rescue erythroid maturation, we propose that SetD8 regulation of erythroid maturation involves multiple target genes. These results establish SetD8 as a determinant of erythroid cell maturation and provide a framework for understanding how a broadly expressed histone-modifying enzyme mediates cell-type-specific GATA factor function."xsd:string |
| http://purl.uniprot.org/citations/25855754 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.01422-14"xsd:string |
| http://purl.uniprot.org/citations/25855754 | http://purl.uniprot.org/core/author | "Hall B.D."xsd:string |
| http://purl.uniprot.org/citations/25855754 | http://purl.uniprot.org/core/author | "Bresnick E.H."xsd:string |
| http://purl.uniprot.org/citations/25855754 | http://purl.uniprot.org/core/author | "Sanalkumar R."xsd:string |
| http://purl.uniprot.org/citations/25855754 | http://purl.uniprot.org/core/author | "DeVilbiss A.W."xsd:string |
| http://purl.uniprot.org/citations/25855754 | http://purl.uniprot.org/core/author | "Katsumura K.R."xsd:string |
| http://purl.uniprot.org/citations/25855754 | http://purl.uniprot.org/core/author | "de Andrade I.F."xsd:string |
| http://purl.uniprot.org/citations/25855754 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25855754 | http://purl.uniprot.org/core/name | "Mol Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/25855754 | http://purl.uniprot.org/core/pages | "2073-2087"xsd:string |
| http://purl.uniprot.org/citations/25855754 | http://purl.uniprot.org/core/title | "Epigenetic Determinants of Erythropoiesis: Role of the Histone Methyltransferase SetD8 in Promoting Erythroid Cell Maturation and Survival."xsd:string |
| http://purl.uniprot.org/citations/25855754 | http://purl.uniprot.org/core/volume | "35"xsd:string |
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