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http://purl.uniprot.org/citations/25858550http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/25858550http://www.w3.org/2000/01/rdf-schema#comment"

Objective

Transforming growth factor-beta (TGF-β) proteins are involved in epithelial keratinization. The major function of latent TGF-β binding proteins (LTBPs) is modulating TGF-β activity. However, whether LTBP-1 and LTBP-2 play roles in gingiva keratinization remains unclear.

Materials and methods

Human keratinized gingiva and non-keratinized alveolar mucosa were processed for LTBP-1, LTBP-2, cytokeratin-1 (K1), cytokeratin-4 (K4), and TGF-β immunohistochemical (IHC) staining. Porcine heterotopically transplanted connective tissues and newly grown epithelia were harvested for IHC staining. The expression levels of LTBP-1 and LTBP-2 were compared between differentiated and undifferentiated human normal oral keratinocytes (hNOK). The expression of LTBP-1 and LTBP-2 was knocked down in a cell line (OEC-M1) to evaluate the effects on the expression of K1, K4, and involucrin (INV).

Results

In human and porcine specimens, LTBP-2 expression patterns distinguished keratinized and non-keratinized oral epithelia. Western blotting results showed that K1, LTBP-1, and INV proteins were upregulated in differentiated hNOK. In OEC-M1 cells, LTBP-2 knockdown resulted in upregulated the expression of K1 and INV and downregulated the expression of K4. LTBP-1 knockdown resulted in opposite effects.

Conclusion

The expression patterns of LTBP-2 differ in keratinized gingiva and non-keratinized mucosa. LTBP-1 and LTBP-2 are involved in the keratinization of oral epithelium; however, the underlying mechanism remains to be elucidated."xsd:string
http://purl.uniprot.org/citations/25858550http://purl.org/dc/terms/identifier"doi:10.1111/odi.12344"xsd:string
http://purl.uniprot.org/citations/25858550http://purl.uniprot.org/core/author"Hsu C.W."xsd:string
http://purl.uniprot.org/citations/25858550http://purl.uniprot.org/core/author"Yang J.R."xsd:string
http://purl.uniprot.org/citations/25858550http://purl.uniprot.org/core/author"Yuan K."xsd:string
http://purl.uniprot.org/citations/25858550http://purl.uniprot.org/core/author"Hsu C.C."xsd:string
http://purl.uniprot.org/citations/25858550http://purl.uniprot.org/core/author"Liao S.C."xsd:string
http://purl.uniprot.org/citations/25858550http://purl.uniprot.org/core/author"Chiang M.S."xsd:string
http://purl.uniprot.org/citations/25858550http://purl.uniprot.org/core/date"2015"xsd:gYear
http://purl.uniprot.org/citations/25858550http://purl.uniprot.org/core/name"Oral Dis"xsd:string
http://purl.uniprot.org/citations/25858550http://purl.uniprot.org/core/pages"762-769"xsd:string
http://purl.uniprot.org/citations/25858550http://purl.uniprot.org/core/title"Latent transforming growth factor-beta binding proteins (LTBP-1 and LTBP-2) and gingiva keratinization."xsd:string
http://purl.uniprot.org/citations/25858550http://purl.uniprot.org/core/volume"21"xsd:string
http://purl.uniprot.org/citations/25858550http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/25858550
http://purl.uniprot.org/citations/25858550http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/25858550
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http://purl.uniprot.org/uniprot/#_Q59EE6-mappedCitation-25858550http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/25858550
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