| http://purl.uniprot.org/citations/2586524 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2586524 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2586524 | http://www.w3.org/2000/01/rdf-schema#comment | "The metallothionein-A gene in the metallothionein gene family of the sea urchin Strongylocentrotus purpuratus (SpMTA gene) was sequenced and found to contain three coding exons plus a 3' entirely noncoding exon. Putative alpha and beta MT domains were encoded, by its exons 2 and 3, respectively, in reverse of the order in vertebrate metallothionein genes. The SpMTA promoter was characterized through the expression of recombinant constructs containing various portions of the proximal 678-base-pair (bp) 5'-flanking region of the SpMTA gene. Zygotes injected with constructs were cultured to the blastula stage in the presence of a heavy-metal chelator and then incubated in the presence or absence of cadmium. The longest constructs were expressed only when heavy-metal ion was present. Two putative metal-responsive elements (MREs a and b) within 240 bp of the transcription start site resembled mammalian MREs in their critical 8-bp cores (TGCRCNCS) and in their locations relative to each other and to the TATA box. Elimination of activity by site-specific mutations in MREs a and b, separately or in both, identified them as metal regulatory elements. Thus, MRE recognition in this invertebrate resembles that in vertebrates. Upstream sites with single-mismatched MREs neither acted as MREs nor amplified the activity of MREs a and b. The SpMTA, Spec1, and CyIIIa actin genes, which have the same ectodermal specificity, have common DNA elements at relatively similar locations in their promoter regions; however, these elements are insufficient in themselves to promote gene expression."xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.9.12.5445-5455.1989"xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.9.12.5445-5455.1989"xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/author | "Nemer M."xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/author | "Nemer M."xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/author | "Harlow P."xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/author | "Harlow P."xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/author | "Thornton R.D."xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/author | "Thornton R.D."xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/author | "Watkins E."xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/author | "Watkins E."xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/pages | "5445-5455"xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/pages | "5445-5455"xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/title | "Structure of an ectodermally expressed sea urchin metallothionein gene and characterization of its metal-responsive region."xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/title | "Structure of an ectodermally expressed sea urchin metallothionein gene and characterization of its metal-responsive region."xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/2586524 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2586524 |
| http://purl.uniprot.org/citations/2586524 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2586524 |