| http://purl.uniprot.org/citations/25867090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25867090 | http://www.w3.org/2000/01/rdf-schema#comment | "Determining the mode of action of bioactive chemicals is of interest to a broad range of academic, pharmaceutical, and industrial scientists. Saccharomyces cerevisiae, or budding yeast, is a model eukaryote for which a complete collection of ~6,000 gene deletion mutants and hypomorphic essential gene mutants are commercially available. These collections of mutants can be used to systematically detect chemical-gene interactions, i.e. genes necessary to tolerate a chemical. This information, in turn, reports on the likely mode of action of the compound. Here we describe a protocol for the rapid identification of chemical-genetic interactions in budding yeast. We demonstrate the method using the chemotherapeutic agent 5-fluorouracil (5-FU), which has a well-defined mechanism of action. Our results show that the nuclear TRAMP RNA exosome and DNA repair enzymes are needed for proliferation in the presence of 5-FU, which is consistent with previous microarray based bar-coding chemical genetic approaches and the knowledge that 5-FU adversely affects both RNA and DNA metabolism. The required validation protocols of these high-throughput screens are also described."xsd:string |
| http://purl.uniprot.org/citations/25867090 | http://purl.org/dc/terms/identifier | "doi:10.3791/52345"xsd:string |
| http://purl.uniprot.org/citations/25867090 | http://purl.uniprot.org/core/author | "Nelson C.J."xsd:string |
| http://purl.uniprot.org/citations/25867090 | http://purl.uniprot.org/core/author | "Dilworth D."xsd:string |
| http://purl.uniprot.org/citations/25867090 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25867090 | http://purl.uniprot.org/core/name | "J Vis Exp"xsd:string |
| http://purl.uniprot.org/citations/25867090 | http://purl.uniprot.org/core/pages | "e52345"xsd:string |
| http://purl.uniprot.org/citations/25867090 | http://purl.uniprot.org/core/title | "Rapid identification of chemical genetic interactions in Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/25867090 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25867090 |
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| http://purl.uniprot.org/uniprot/#_P06778-mappedCitation-25867090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25867090 |
| http://purl.uniprot.org/uniprot/#_P07280-mappedCitation-25867090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25867090 |
| http://purl.uniprot.org/uniprot/#_P12753-mappedCitation-25867090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25867090 |
| http://purl.uniprot.org/uniprot/#_P40507-mappedCitation-25867090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25867090 |
| http://purl.uniprot.org/uniprot/#_P48561-mappedCitation-25867090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25867090 |
| http://purl.uniprot.org/uniprot/#_Q06164-mappedCitation-25867090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25867090 |
| http://purl.uniprot.org/uniprot/#_P32829-mappedCitation-25867090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25867090 |
| http://purl.uniprot.org/uniprot/P48561 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25867090 |
| http://purl.uniprot.org/uniprot/P06778 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25867090 |
| http://purl.uniprot.org/uniprot/P07280 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25867090 |
| http://purl.uniprot.org/uniprot/P12753 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25867090 |
| http://purl.uniprot.org/uniprot/P32829 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25867090 |
| http://purl.uniprot.org/uniprot/Q12149 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25867090 |