| http://purl.uniprot.org/citations/25872469 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25872469 | http://www.w3.org/2000/01/rdf-schema#comment | "The aim of the present study was to produce the human dual specificity phosphatase 1 (DUSP1) protein with biological activity and to investigate its in vitro effects on cancer cells. DUSP1 protein was expressed in the baculovirus expression system and purified by Ni-affinity chromatography followed by dialysis in PBS. The purified protein was verified by SDS-PAGE and western blot analysis. Six cancer cell lines were then cultured in the presence of DUSP1 for various periods of time, and the phosphorylated extracellular signal-regulated kinase (p-ERK) content in each cell line was subsequently determined by western blot analysis. Compared to the β-actin level, the amount of p-ERK markedly decreased after 1 h, indicating that DUSP1 suppressed the expression of p-ERK in 6 cancer cell lines examined. Human cervical cancer cells were also collected and counted following co-culture with DUSP1 to examine its effect on the growth rate of cancer cells. A baculovirus expression system for the production of DUSP1 protein was successfully constructed. The p-ERK content was found to be significantly decreased when the cancer cell lines were exposed to DUSP1. The capability of binary fission was reduced when the cells were examined under a microscope. The proliferation of human cervical cancer cells was also inhibited by DUSP1."xsd:string |
| http://purl.uniprot.org/citations/25872469 | http://purl.org/dc/terms/identifier | "doi:10.3892/ijmm.2015.2179"xsd:string |
| http://purl.uniprot.org/citations/25872469 | http://purl.uniprot.org/core/author | "Huang L."xsd:string |
| http://purl.uniprot.org/citations/25872469 | http://purl.uniprot.org/core/author | "Hong Y."xsd:string |
| http://purl.uniprot.org/citations/25872469 | http://purl.uniprot.org/core/author | "Zhu S."xsd:string |
| http://purl.uniprot.org/citations/25872469 | http://purl.uniprot.org/core/author | "Jun L."xsd:string |
| http://purl.uniprot.org/citations/25872469 | http://purl.uniprot.org/core/author | "Cheng P."xsd:string |
| http://purl.uniprot.org/citations/25872469 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25872469 | http://purl.uniprot.org/core/name | "Int J Mol Med"xsd:string |
| http://purl.uniprot.org/citations/25872469 | http://purl.uniprot.org/core/pages | "1715-1719"xsd:string |
| http://purl.uniprot.org/citations/25872469 | http://purl.uniprot.org/core/title | "Production of DUSP1 protein using the baculovirus insect cell expression system and its in vitro effects on cancer cells."xsd:string |
| http://purl.uniprot.org/citations/25872469 | http://purl.uniprot.org/core/volume | "35"xsd:string |
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